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Literature summary for 2.1.1.283 extracted from

  • Chen, Z.G.; Fujii, I.; Ebizuka, Y.; Sankawa, U.
    Emodin O-methyltransferase from Aspergillus terreus (1992), Arch. Microbiol., 158, 29-34.
    View publication on PubMed
Search for more literature for 2.1.1.283

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzene sulfonate potent inhibition Aspergillus terreus
iodoacetate slight inhibition Aspergillus terreus
additional information no inhibition by metal chelating agents Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00034
-
 emodin pH 8.0, 30°C Aspergillus terreus
0.0041
-
 S-adenosyl-L-methionine pH 8.0, 30°C Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ or Mn2+ is not an absolute requirement, nor increases the enzyme activity Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53600
-
 6 * 53600, SDS-PAGE Aspergillus terreus
322000
-
 gel filtration Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + emodin Aspergillus terreus the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin S-adenosyl-L-homocysteine + questin
-
 ?
S-adenosyl-L-methionine + emodin Aspergillus terreus IMI 16043 the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin S-adenosyl-L-homocysteine + questin
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus IMI 16043
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus terreus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus terreus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.18
-
 pH 8.0, 30°C Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone i.e. citreorosein, 22% of the activity compared to emodin Aspergillus terreus ?
-
 ?
S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone i.e. citreorosein, 22% of the activity compared to emodin Aspergillus terreus IMI 16043 ?
-
 ?
S-adenosyl-L-methionine + 7-chloroemodin 18% of the activity compared to emodin Aspergillus terreus ?
-
 ?
S-adenosyl-L-methionine + 7-chloroemodin 18% of the activity compared to emodin Aspergillus terreus IMI 16043 ?
-
 ?
S-adenosyl-L-methionine + catenarin 80% of the activity compared to emodin Aspergillus terreus ?
-
 ?
S-adenosyl-L-methionine + catenarin 80% of the activity compared to emodin Aspergillus terreus IMI 16043 ?
-
 ?
S-adenosyl-L-methionine + emodin the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin Aspergillus terreus S-adenosyl-L-homocysteine + questin
-
 ?
S-adenosyl-L-methionine + emodin
-
 Aspergillus terreus S-adenosyl-L-homocysteine + questin
-
 ?
S-adenosyl-L-methionine + emodin the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin Aspergillus terreus IMI 16043 S-adenosyl-L-homocysteine + questin
-
 ?
S-adenosyl-L-methionine + emodin
-
 Aspergillus terreus IMI 16043 S-adenosyl-L-homocysteine + questin
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 53600, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
EOMT
-
 Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Aspergillus terreus

pH Range

pH Minimum pH Maximum Comment Organism
5.7 9 half maximal activity at pH 5.7 and pH 9.0 Aspergillus terreus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus terreus isoelectric focusing
-
 4.4