Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme | Nanoarchaeum equitans |
recombinant expression of His-tagged enzyme | Saccharolobus solfataricus |
recombinant expression of His-tagged wild-type and mutant enzymes | Pyrococcus abyssi |
Protein Variants | Comment | Organism |
---|---|---|
E173A | site-directed mutagenesis, the mutant shows 9 and 26% of wild-type activity for imG and imG2 formation, respectively | Pyrococcus abyssi |
E213A | site-directed mutagenesis, inactive mutant | Pyrococcus abyssi |
F165A | site-directed mutagenesis, inactive mutant | Pyrococcus abyssi |
P260N | site-directed mutagenesis, the mutant shows no and 114% of wild-type activity for imG and imG2 formation, respectively | Pyrococcus abyssi |
P262A | site-directed mutagenesis, the mutant shows 5 and 8% of wild-type activity for imG and imG2 formation, respectively | Pyrococcus abyssi |
R134A | site-directed mutagenesis, the mutant shows 2 and 4% of wild-type activity for imG and imG2 formation, respectively | Pyrococcus abyssi |
R174A | site-directed mutagenesis, the mutant shows 8 and 69% of wild-type activity for imG and imG2 formation, respectively | Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pyrococcus abyssi | |
Mg2+ | required | Nanoarchaeum equitans | |
Mg2+ | required | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 4-demethylwyosine | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | Nanoarchaeum equitans | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | Saccharolobus solfataricus | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | Saccharolobus solfataricus P2 | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | Saccharolobus solfataricus JCM 11322 | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | Saccharolobus solfataricus ATCC 35092 | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | Saccharolobus solfataricus DSM 1617 | - |
S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + wyosine | - |
? | |
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine | Nanoarchaeum equitans | - |
S-adenosyl-L-homocysteine + wyosine | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Nanoarchaeum equitans | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Saccharolobus solfataricus | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Saccharolobus solfataricus P2 | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Saccharolobus solfataricus JCM 11322 | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Saccharolobus solfataricus ATCC 35092 | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | Saccharolobus solfataricus DSM 1617 | - |
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nanoarchaeum equitans | Q74NE4 | - |
- |
Pyrococcus abyssi | Q9V2G1 | - |
- |
Pyrococcus abyssi Orsay | Q9V2G1 | - |
- |
Saccharolobus solfataricus | Q97W08 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus ATCC 35092 | Q97W08 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus DSM 1617 | Q97W08 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus JCM 11322 | Q97W08 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus P2 | Q97W08 | i.e. Sulfolobus solfataricus | - |
Purification (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme by affinity chromatography and desalting gel filtration | Nanoarchaeum equitans |
recombinant expression of His-tagged enzyme by affinity chromatography and desalting gel filtration | Saccharolobus solfataricus |
recombinant expression of His-tagged wild-type and mutant enzymes to near homogeneity by 20 min heat treatment at 70°C and 65°C, respectively, followed by affinity chromatography and desalting gel filtration | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nanoarchaeum equitans NEQ228 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading to the formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant | Nanoarchaeum equitans | ? | - |
- |
|
additional information | Pyrococcus abyssi PAB2272 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading to the formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant | Pyrococcus abyssi | ? | - |
- |
|
additional information | Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant | Saccharolobus solfataricus | ? | - |
- |
|
additional information | Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant | Saccharolobus solfataricus P2 | ? | - |
- |
|
additional information | Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant | Saccharolobus solfataricus JCM 11322 | ? | - |
- |
|
additional information | Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant | Saccharolobus solfataricus ATCC 35092 | ? | - |
- |
|
additional information | Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant | Saccharolobus solfataricus DSM 1617 | ? | - |
- |
|
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Nanoarchaeum equitans | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Saccharolobus solfataricus | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Pyrococcus abyssi | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Nanoarchaeum equitans | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Saccharolobus solfataricus | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Saccharolobus solfataricus P2 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Saccharolobus solfataricus P2 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Saccharolobus solfataricus JCM 11322 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Saccharolobus solfataricus JCM 11322 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Saccharolobus solfataricus ATCC 35092 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Saccharolobus solfataricus ATCC 35092 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | - |
Saccharolobus solfataricus DSM 1617 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine | i.e. im-G14, activity of EC 2.1.1.282 | Saccharolobus solfataricus DSM 1617 | S-adenosyl-L-homocysteine + isowyosine | - |
? | |
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + wyosine | - |
? | |
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine | - |
Nanoarchaeum equitans | S-adenosyl-L-homocysteine + wyosine | - |
? | |
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine | i.e. yW-86, activity of EC 2.1.1.228 | Pyrococcus abyssi | S-adenosyl-L-homocysteine + wyosine | - |
? | |
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine | i.e. yW-86, activity of EC 2.1.1.228 | Nanoarchaeum equitans | S-adenosyl-L-homocysteine + wyosine | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Nanoarchaeum equitans | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Saccharolobus solfataricus | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Saccharolobus solfataricus P2 | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Saccharolobus solfataricus JCM 11322 | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Saccharolobus solfataricus ATCC 35092 | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe | - |
Saccharolobus solfataricus DSM 1617 | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aTrm5a methyltransferase | - |
Pyrococcus abyssi |
aTrm5a methyltransferase | - |
Nanoarchaeum equitans |
aTrm5a methyltransferase | - |
Saccharolobus solfataricus |
aTrm5a-type enzyme | - |
Saccharolobus solfataricus |
aTrm5a/Taw22-like enzyme | - |
Pyrococcus abyssi |
More | see also EC 2.1.1.228 | Pyrococcus abyssi |
More | see also EC 2.1.1.228 | Nanoarchaeum equitans |
NEQ228 | - |
Nanoarchaeum equitans |
PAB2272 | - |
Pyrococcus abyssi |
SSO2439 | - |
Saccharolobus solfataricus |
Taw21 | - |
Saccharolobus solfataricus |
TAW22 | - |
Pyrococcus abyssi |
TAW22 | - |
Nanoarchaeum equitans |
Trm5a | - |
Pyrococcus abyssi |
Trm5a | - |
Nanoarchaeum equitans |
Trm5a/Taw22-like enzyme | - |
Nanoarchaeum equitans |
tRNAPhe:imG2 methyltransferase | - |
Pyrococcus abyssi |
tRNAPhe:imG2 methyltransferase | - |
Nanoarchaeum equitans |
tRNAPhe:imG2 methyltransferase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrococcus abyssi |
50 | - |
assay at | Nanoarchaeum equitans |
50 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pyrococcus abyssi |
8 | - |
assay at | Nanoarchaeum equitans |
8 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Pyrococcus abyssi | |
S-adenosyl-L-methionine | - |
Nanoarchaeum equitans | |
S-adenosyl-L-methionine | - |
Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
evolution | archaeal Trm5a, a member of the archaeal Trm5a/b/c family of enzymes involved in the biosynthesis of the wyosine derivatives, division of the family aTrm5 into three subfamilies aTrm5a (further divided into Taw21 and Taw22 which are monofunctional and bifunctional aTrm5a ), aTrm5b, and aTrm5c. While the enzymes belonging to these subfamilies do not significantly differ in their AdoMet-binding site, small differences have been observed within the NPPY motif, which, in certain amino-methyltransferases, is involved in the positioning of the target nitrogen atom. In contrast, the N-terminal sequences of the aforementioned enzymes differ substantially, e.g. a small conservative domain called D1 is present in aTrm5b and aTrm5c but absent in most of the aTrm5a proteins. Evolution of tRNAPhe:imG2 methyltransferases involved in the biosynthesis of wyosine derivatives in Archaea. Amino acid sequence alignment of Trm5a/b/c/ family of proteins. Monofunctional and bifunctional aTrm5a enzymes, overview | Nanoarchaeum equitans |
evolution | archaeal Trm5a, a member of the archaeal Trm5a/b/c family of enzymes involved in the biosynthesis of the wyosine derivatives, division of the family aTrm5 into three subfamilies aTrm5a (further divided into Taw21 and Taw22 which are monofunctional and bifunctional aTrm5a ), aTrm5b, and aTrm5c. While the enzymes belonging to these subfamilies do not significantly differ in their AdoMet-binding site, small differences have been observed within the NPPY motif, which, in certain amino-methyltransferases, is involved in the positioning of the target nitrogen atom. In contrast, the N-terminal sequences of the aforementioned enzymes differ substantially, e.g. a small conservative domain called D1 is present in aTrm5b and aTrm5c but absent in most of the aTrm5a proteins. Evolution of tRNAPhe:imG2 methyltransferases involved in the biosynthesis of wyosine derivatives in Archaea. Amino acid sequence alignment of Trm5a/b/c/ family of proteins. Monofunctional and bifunctional aTrm5a enzymes, overview. Crenarcheota Saccharolobus solfataricus as well as in other Sulfalobales and Desulfurococcales, two different tRNAPhe methyltransferases are involved in the biosynthesis of mimG, catalyzing the formation of m1G (Trm5c, EC 2.1.1.228) and imG2 (Trm5a, EC 2.1.1.282) at position 37 in tRNAPhe, respectively | Saccharolobus solfataricus |
evolution | archaeal Trm5a, a member of the archaeal Trm5a/b/c family of enzymes involved in the biosynthesis of the wyosine derivatives, division of the family aTrm5 into three subfamilies aTrm5a (further divided into Taw21 and Taw22 which are monofunctional and bifunctional aTrm5a), aTrm5b, and aTrm5c. While the enzymes belonging to these subfamilies do not significantly differ in their AdoMet-binding site, small differences have been observed within the NPPY motif, which, in certain amino-methyltransferases, is involved in the positioning of the target nitrogen atom. In contrast, the N-terminal sequences of the aforementioned enzymes differ substantially, e.g. a small conservative domain called D1 is present in aTrm5b and aTrm5c but absent in most of the aTrm5a proteins. Evolution of tRNAPhe:imG2 methyltransferases involved in the biosynthesis of wyosine derivatives in Archaea. Amino acid sequence alignment of Trm5a/b/c/ family of proteins. Monofunctional and bifunctional aTrm5a enzymes, overview | Pyrococcus abyssi |
malfunction | substitutions of individual conservative amino acids of Pyrococcus abyssi Taw22 (P260N, E173A, and R174A) have a differential effect on the formation of m1G/imG2, while replacement of R134, F165, E213, and P262 with alanine abolishes the formation of both derivatives of G37 | Pyrococcus abyssi |
malfunction | substitutions of individual conservative amino acids of Pyrococcus abyssi Taw22 (P260N, E173A, and R174A) have a differential effect on the formation of m1G/imG2, while replacement of R134, F165, E213, and P262 with alanine abolishes the formation of both derivatives of G37 | Nanoarchaeum equitans |
malfunction | substitutions of individual conservative amino acids of Pyrococcus abyssi Taw22 (P260N, E173A, and R174A) have a differential effect on the formation of m1G/imG2, while replacement of R134, F165, E213, and P262 with alanine abolishes the formation of both derivatives of G37 | Saccharolobus solfataricus |
metabolism | putative enzymatic pathway leading to the formation of wyosine derivatives in Archaea | Pyrococcus abyssi |
metabolism | putative enzymatic pathway leading to the formation of wyosine derivatives in Archaea | Nanoarchaeum equitans |
metabolism | putative enzymatic pathway leading to the formation of wyosine derivatives in Archaea | Saccharolobus solfataricus |
physiological function | tricyclic wyosine derivatives are found at position 37 of eukaryotic and archaeal tRNAPhe. In Archaea, the intermediate imG-14 is targeted by three different enzymes that catalyze the formation of yW-86, imG, and imG2. Methyltransferase aTrm5a/Taw22 likely catalyzes two distinct reactions: N1-methylation of guanosine to yield m1G (EC 2.1.1.228), and C7-methylation of imG-14 to yield imG2 (EC 2.1.1.282) | Pyrococcus abyssi |
physiological function | tricyclic wyosine derivatives are found at position 37 of eukaryotic and archaeal tRNAPhe. In Archaea, the intermediate imG-14 is targeted by three different enzymes that catalyze the formation of yW-86, imG, and imG2. Methyltransferase aTrm5a/Taw22 likely catalyzes two distinct reactions: N1-methylation of guanosine to yield m1G (EC 2.1.1.228), and C7-methylation of imG-14 to yield imG2 (EC 2.1.1.282) | Nanoarchaeum equitans |
physiological function | tricyclic wyosine derivatives are found at position 37 of eukaryotic and archaeal tRNAPhe. In Archaea, the intermediate imG-14 is targeted by three different enzymes that catalyze the formation of yW-86, imG, and imG2. Methyltransferase aTrm5a/Taw22 likely catalyzes two distinct reactions: N1-methylation of guanosine to yield m1G (EC 2.1.1.228), and C7-methylation of imG-14 to yield imG2 (EC 2.1.1.282) | Saccharolobus solfataricus |