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Literature summary for 2.1.1.282 extracted from

  • Noma, A.; Kirino, Y.; Ikeuchi, Y.; Suzuki, T.
    Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA (2006), EMBO J., 25, 2142-2154.
    View publication on PubMed
Search for more literature for 2.1.1.282

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine-tagged enzyme is expressed in Saccharomyces cerevisiae Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe Saccharomyces cerevisiae the enzyme is involved in the biosynthesis of the tricyclic base wybutosine S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-methyl]wyosine37 in tRNAPhe
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53177
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe the enzyme is involved in the biosynthesis of the tricyclic base wybutosine Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-methyl]wyosine37 in tRNAPhe
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 ?

Synonyms

Synonyms Comment Organism
TYW3
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 Saccharomyces cerevisiae
YGL050w
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 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction in DELTATYW3 strain, tRNAPhe has two wybutosine intermediates, 7-(3-amino-3-carboxypropyl)-4-demethylwyosine37 (yW-86) and 4-demethylwyosine37 (yW-14). Recombinant TYW3 clearly methylated 7-(3-amino-3-carboxypropyl)-4-demethylwyosine37 in tRNA to yield 7-(3-amino-3-carboxypropyl)-4-methylwyosine37 in tRNA in S-adenosyl-L-methionine-dependent manner. 4-Demethylwyosine37 is not methylated by TYW3 Saccharomyces cerevisiae
physiological function the enzyme is involved in the biosynthesis of the tricyclic base wybutosine Saccharomyces cerevisiae