Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-selenocysteine | above 1 mM | Astragalus bisulcatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
S-adenosyl-L-methionine | pH 6.0, 30°C | Astragalus bisulcatus | |
0.3 | - |
DL-selenocysteine | pH 6.0, 30°C | Astragalus bisulcatus | |
0.7 | - |
L-selenocysteine | pH 6.0, 30°C | Astragalus bisulcatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Astragalus bisulcatus |
38000 | - |
SDS-PAGE and gel filtration on a Superdex 75 column | Astragalus bisulcatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + S-adenosyl-L-methionine | Astragalus bisulcatus | the enzyme is suitable for specific detoxification of selenocysteine and preventing selenium from intrusion into downstream sulfur pathways. Plays a crucial role in conferring selenium tolerance | Se-methyl-L-selenocysteine + S-adenosyl-L-homocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | Astragalus bisulcatus | - |
L-methionine + Se-methyl-L-selenocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Astragalus bisulcatus | P56707 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Astragalus bisulcatus |
more than 2800fold purification by (NH4)2SO4 precipitation, anion-exchange chromatography and hydrophobic-interaction chromatography | Astragalus bisulcatus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00971 | - |
- |
Astragalus bisulcatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-selenocysteine + S-adenosyl-L-methionine | the stereochemical orientation of the alpha-amino group is not crucial. With DL-selenocysteine or DL-selenohomocysteine as substrates, the Km decreases. This leads to the overproportional increase of specific activity with DL-selenocysteine | Astragalus bisulcatus | Se-methyl-selenocysteine + S-adenosyl-L-homocysteine | - |
? | |
L-selenocysteine + S-adenosyl-L-methionine | the enzyme is suitable for specific detoxification of selenocysteine and preventing selenium from intrusion into downstream sulfur pathways. Plays a crucial role in conferring selenium tolerance | Astragalus bisulcatus | Se-methyl-L-selenocysteine + S-adenosyl-L-homocysteine | - |
? | |
additional information | methylation of L-cysteine can not be detected unless the concentration is raised to 10 mM. Under this condition, the reaction velocity is approximately 1% compared with methylation of L-selenocysteine. No activity with selenocysteamine and DL-homocysteine | Astragalus bisulcatus | ? | - |
? | |
S-adenosyl-L-methionine + DL-homocysteine | less than 1% relative activity with 10 mM DL-homocysteine compared to activity with 1 mM DL-selenocysteine set at 100% | Astragalus bisulcatus | ? | - |
? | |
S-adenosyl-L-methionine + DL-selenocysteine | 475% relative activity with 1 mM DL-selenocysteine compared to 1 mM L-selenocysteine activity set at 100% | Astragalus bisulcatus | ? | - |
? | |
S-adenosyl-L-methionine + DL-selenohomocysteine | 25% relative activity with 1 mM DL-selenohomocysteine compared to activity with 1 mM DL-selenocysteine set at 100% | Astragalus bisulcatus | ? | - |
? | |
S-adenosyl-L-methionine + L-cysteine | 1% relative activity with 10 mM L-cysteine compared to 1 mM L-selenocysteine activity set at 100% | Astragalus bisulcatus | S-methyl-L-cysteine + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + L-selenocysteine | - |
Astragalus bisulcatus | Se-methyl-L-selenocysteine + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + selenocysteamine | less than 1% relative activity with 1 mM selenocysteamine compared to 1 mM L-selenocysteine activity set at 100% | Astragalus bisulcatus | ? | - |
? | |
S-methyl-L-methionine + L-selenocysteine | - |
Astragalus bisulcatus | L-methionine + Se-methyl-L-selenocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Astragalus bisulcatus |
Synonyms | Comment | Organism |
---|---|---|
SEcMT | - |
Astragalus bisulcatus |
selenocysteine methyltransferase | - |
Astragalus bisulcatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Astragalus bisulcatus |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme plays a crucial role in the detoxification of selenium in Astragalus bisulcatus | Astragalus bisulcatus |