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Literature summary for 2.1.1.276 extracted from

  • Varbanova, M.; Yamaguchi, S.; Yang, Y.; McKelvey, K.; Hanada, A.; Borochov, R.; Yu, F.; Jikumaru, Y.; Ross, J.; Cortes, D.; Ma, C.J.; Noel, J.P.; Mander, L.; Shulaev, V.; Kamiya, Y.; Rodermel, S.; Weiss, D.; Pichersky, E.
    Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2 (2007), Plant Cell, 19, 32-45.
    View publication on PubMed
Search for more literature for 2.1.1.276

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
Fe3+ 5 mM, more than 90% inhibition Arabidopsis thaliana
Zn2+ 5 mM, more than 90% inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana
0.0059
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
 x * 43300, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + gibberellin A4 Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + methyl gibberellin A4
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q5XF78
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Arabidopsis thaliana
-
silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with gibberelin A12 and gibberelin A19 Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + gibberellin A1 30% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A1
-
 ?
S-adenosyl-L-methionine + gibberellin A20 15% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A20
-
 ?
S-adenosyl-L-methionine + gibberellin A3 45% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A3
-
 ?
S-adenosyl-L-methionine + gibberellin A34 80% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A34
-
 ?
S-adenosyl-L-methionine + gibberellin A4
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A4
-
 ?
S-adenosyl-L-methionine + gibberellin A51 16% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A51
-
 ?
S-adenosyl-L-methionine + gibberellin A9 60% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A9
-
 ?

Subunits

Subunits Comment Organism
? x * 43300, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GAMT2
-
 Arabidopsis thaliana
gibberellin A4 O-methyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
0.0018
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development up

General Information

General Information Comment Organism
malfunction GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol Arabidopsis thaliana
physiological function methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
0.9
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana