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Literature summary for 2.1.1.275 extracted from

  • Varbanova, M.; Yamaguchi, S.; Yang, Y.; McKelvey, K.; Hanada, A.; Borochov, R.; Yu, F.; Jikumaru, Y.; Ross, J.; Cortes, D.; Ma, C.J.; Noel, J.P.; Mander, L.; Shulaev, V.; Kamiya, Y.; Rodermel, S.; Weiss, D.; Pichersky, E.
    Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2 (2007), Plant Cell, 19, 32-45.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 5 mM, 4fold activation Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, expression in Arabidopsis under the control of the 35S cauliflower mosaic virus promoter Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
Fe2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
Fe3+ 5 mM, more than 90% inhibition Arabidopsis thaliana
Zn2+ 5 mM, more than 90% inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
0.0158
-
gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5 mM, 3fold activation Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Arabidopsis thaliana
41500
-
x * 41500, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + gibberellin A9 Arabidopsis thaliana methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature S-adenosyl-L-homocysteine + methyl gibberellin A9
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4JUY5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed GAMT1 expression is localized mostly to seed Arabidopsis thaliana
-
silique expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + gibberellin 1 25% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A1
-
?
S-adenosyl-L-methionine + gibberellin A19 19% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A19
-
?
S-adenosyl-L-methionine + gibberellin A20 95% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A20
-
?
S-adenosyl-L-methionine + gibberellin A3 80% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A3
-
?
S-adenosyl-L-methionine + gibberellin A34 46% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A34
-
?
S-adenosyl-L-methionine + gibberellin A4 69% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A4
-
?
S-adenosyl-L-methionine + gibberellin A51 32% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A51
-
?
S-adenosyl-L-methionine + gibberellin A9 methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A9
-
?
S-adenosyl-L-methionine + gibberellin A9 gibberellin A9 i.e. (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylic acid Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A9 methyl gibberellin A9 i.e. methyl (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylate ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Arabidopsis thaliana
? x * 41500, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GAMT1
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
0.026
-
gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development up

General Information

General Information Comment Organism
malfunction GAMT1 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol Arabidopsis thaliana
physiological function methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana
1.85
-
gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana