BRENDA - Enzyme Database
show all sequences of 2.1.1.274

A novel methyltransferase from the intracellular pathogen Plasmodiophora brassicae methylates salicylic acid

Ludwig-Mueller, J.; Juelke, S.; Geiss, K.; Richter, F.; Mithoefer, A.; ?ola, I.; Rusak, G.; Keenan, S.; Bulman, S.; Mol. Plant Pathol. 16, 349-364 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus
Plasmodiophora brassicae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Plasmodiophora brassicae
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
?
-
-
-
S-adenosyl-L-methionine + salicylate
Plasmodiophora brassicae
-
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Plasmodiophora brassicae
R4I7S9
gene PbBSMT
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography
Plasmodiophora brassicae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.009
-
purified recombinant enzyme, pH and temperature not specified in the publication
Plasmodiophora brassicae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
736842
Plasmodiophora brassicae
?
-
-
-
-
S-adenosyl-L-methionine + salicylate
-
736842
Plasmodiophora brassicae
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
benzoic acid/salicylic acid methyltransferase
-
Plasmodiophora brassicae
BSMT
-
Plasmodiophora brassicae
SA methyltransferase
-
Plasmodiophora brassicae
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Plasmodiophora brassicae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus
Plasmodiophora brassicae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Plasmodiophora brassicae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Plasmodiophora brassicae
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
?
-
-
-
S-adenosyl-L-methionine + salicylate
Plasmodiophora brassicae
-
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography
Plasmodiophora brassicae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.009
-
purified recombinant enzyme, pH and temperature not specified in the publication
Plasmodiophora brassicae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
736842
Plasmodiophora brassicae
?
-
-
-
-
S-adenosyl-L-methionine + salicylate
-
736842
Plasmodiophora brassicae
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate
Plasmodiophora brassicae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate
Plasmodiophora brassicae
Other publictions for EC 2.1.1.274
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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736842
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A novel methyltransferase from ...
Plasmodiophora brassicae
Mol. Plant Pathol.
16
349-364
2015
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736841
Zhu
Salicylic acid and jasmonic ac ...
Nicotiana benthamiana
Mol. Plant Microbe Interact.
27
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2014
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726129
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Glycine max
Plant Biotechnol. J.
11
1135-1145
2013
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1
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725781
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Clarkia breweri
J. Phys. Chem. B
115
389-396
2011
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726013
Liu
Altering expression of benzoic ...
Arabidopsis thaliana
Mol. Plant Microbe Interact.
23
82-90
2010
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726172
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Functional analysis of a tomat ...
Solanum lycopersicum
Plant J.
62
113-123
2010
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726132
Kwon
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Biotic and abiotic stresses in ...
Atropa belladonna
Plant Biotechnol.
26
207-215
2009
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723402
Zubieta
Structural basis for substrate ...
Clarkia breweri
Plant Cell
15
1704-1716
2003
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723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
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721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
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721412
Ross
S-Adenosyl-L-methionine:salicy ...
Clarkia breweri
Arch. Biochem. Biophys.
367
9-16
1999
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8
3
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