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Literature summary for 2.1.1.274 extracted from

  • Tieman, D.; Zeigler, M.; Schmelz, E.; Taylor, M.G.; Rushing, S.; Jones, J.B.; Klee, H.J.
    Functional analysis of a tomato salicylic acid methyl transferase and its role in synthesis of the flavor volatile methyl salicylate (2010), Plant J., 62, 113-123.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-AI cells Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0146
-
S-adenosyl-L-methionine in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum
0.05
-
salicylate in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 ?
-
?
S-adenosyl-L-methionine + 4-aminosalicylate Solanum lycopersicum 2% activity compared to salicylate S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
?
S-adenosyl-L-methionine + benzoate Solanum lycopersicum 2% activity compared to salicylate S-adenosyl-L-homocysteine + methyl benzoate
-
?
S-adenosyl-L-methionine + salicylate Solanum lycopersicum 100% activity S-adenosyl-L-homocysteine + methyl salicylate
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bud
-
Solanum lycopersicum
-
flower
-
Solanum lycopersicum
-
fruit
-
Solanum lycopersicum
-
leaf
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 Solanum lycopersicum ?
-
?
S-adenosyl-L-methionine + 4-aminosalicylate 2% activity compared to salicylate Solanum lycopersicum S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
?
S-adenosyl-L-methionine + benzoate 2% activity compared to salicylate Solanum lycopersicum S-adenosyl-L-homocysteine + methyl benzoate
-
?
S-adenosyl-L-methionine + salicylate 100% activity Solanum lycopersicum S-adenosyl-L-homocysteine + methyl salicylate
-
?

Synonyms

Synonyms Comment Organism
salicylic acid methyl transferase
-
Solanum lycopersicum
SAMT
-
Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
S-adenosyl-L-methionine in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum
0.056
-
salicylate in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum

Expression

Organism Comment Expression
Solanum lycopersicum isoform SAMT1 expression drops off to essentially undetectable levels by the time fruits are fully ripened down

General Information

General Information Comment Organism
metabolism the enzyme is critical for methyl salicylate synthesis Solanum lycopersicum