BRENDA - Enzyme Database show
show all sequences of 2.1.1.274

Functional analysis of a tomato salicylic acid methyl transferase and its role in synthesis of the flavor volatile methyl salicylate

Tieman, D.; Zeigler, M.; Schmelz, E.; Taylor, M.G.; Rushing, S.; Jones, J.B.; Klee, H.J.; Plant J. 62, 113-123 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21-AI cells
Solanum lycopersicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0146
-
S-adenosyl-L-methionine
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
0.05
-
salicylate
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Solanum lycopersicum
less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3
?
-
-
-
S-adenosyl-L-methionine + 4-aminosalicylate
Solanum lycopersicum
2% activity compared to salicylate
S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
-
?
S-adenosyl-L-methionine + benzoate
Solanum lycopersicum
2% activity compared to salicylate
S-adenosyl-L-homocysteine + methyl benzoate
-
-
?
S-adenosyl-L-methionine + salicylate
Solanum lycopersicum
100% activity
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bud
-
Solanum lycopersicum
-
flower
-
Solanum lycopersicum
-
fruit
-
Solanum lycopersicum
-
leaf
-
Solanum lycopersicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3
726172
Solanum lycopersicum
?
-
-
-
-
S-adenosyl-L-methionine + 4-aminosalicylate
2% activity compared to salicylate
726172
Solanum lycopersicum
S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
-
-
?
S-adenosyl-L-methionine + benzoate
2% activity compared to salicylate
726172
Solanum lycopersicum
S-adenosyl-L-homocysteine + methyl benzoate
-
-
-
?
S-adenosyl-L-methionine + salicylate
100% activity
726172
Solanum lycopersicum
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.028
-
S-adenosyl-L-methionine
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
0.056
-
salicylate
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-AI cells
Solanum lycopersicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0146
-
S-adenosyl-L-methionine
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
0.05
-
salicylate
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Solanum lycopersicum
less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3
?
-
-
-
S-adenosyl-L-methionine + 4-aminosalicylate
Solanum lycopersicum
2% activity compared to salicylate
S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
-
?
S-adenosyl-L-methionine + benzoate
Solanum lycopersicum
2% activity compared to salicylate
S-adenosyl-L-homocysteine + methyl benzoate
-
-
?
S-adenosyl-L-methionine + salicylate
Solanum lycopersicum
100% activity
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bud
-
Solanum lycopersicum
-
flower
-
Solanum lycopersicum
-
fruit
-
Solanum lycopersicum
-
leaf
-
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3
726172
Solanum lycopersicum
?
-
-
-
-
S-adenosyl-L-methionine + 4-aminosalicylate
2% activity compared to salicylate
726172
Solanum lycopersicum
S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
-
-
?
S-adenosyl-L-methionine + benzoate
2% activity compared to salicylate
726172
Solanum lycopersicum
S-adenosyl-L-homocysteine + methyl benzoate
-
-
-
?
S-adenosyl-L-methionine + salicylate
100% activity
726172
Solanum lycopersicum
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.028
-
S-adenosyl-L-methionine
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
0.056
-
salicylate
in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25C
Solanum lycopersicum
Expression
Organism
Commentary
Expression
Solanum lycopersicum
isoform SAMT1 expression drops off to essentially undetectable levels by the time fruits are fully ripened
down
General Information
General Information
Commentary
Organism
metabolism
the enzyme is critical for methyl salicylate synthesis
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is critical for methyl salicylate synthesis
Solanum lycopersicum
Expression (protein specific)
Organism
Commentary
Expression
Solanum lycopersicum
isoform SAMT1 expression drops off to essentially undetectable levels by the time fruits are fully ripened
down
Other publictions for EC 2.1.1.274
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736189
Wang
Cloning and characterization o ...
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Genet. Mol. Res.
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14510-14521
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1
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1
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2
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736696
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Solanum lycopersicum
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179
122-132
2015
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1
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1
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1
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736842
Ludwig-Mueller
A novel methyltransferase from ...
Plasmodiophora brassicae
Mol. Plant Pathol.
16
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2015
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1
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5
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2
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1
1
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736841
Zhu
Salicylic acid and jasmonic ac ...
Nicotiana benthamiana
Mol. Plant Microbe Interact.
27
567-577
2014
-
-
1
-
1
-
-
-
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1
-
6
-
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1
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1
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1
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1
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1
1
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-
726129
Lin
Overexpression of a soybean sa ...
Glycine max
Plant Biotechnol. J.
11
1135-1145
2013
-
-
1
-
-
-
6
1
-
2
-
5
-
5
-
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1
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5
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1
1
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1
1
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1
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6
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1
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5
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1
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5
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1
1
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1
1
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1
1
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-
725781
Yao
QM/MM free energy simulations ...
Clarkia breweri
J. Phys. Chem. B
115
389-396
2011
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726013
Liu
Altering expression of benzoic ...
Arabidopsis thaliana
Mol. Plant Microbe Interact.
23
82-90
2010
-
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-
-
-
-
-
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5
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2
2
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-
726172
Tieman
Functional analysis of a tomat ...
Solanum lycopersicum
Plant J.
62
113-123
2010
-
-
1
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-
-
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2
-
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4
-
5
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4
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4
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2
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1
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4
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4
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4
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2
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1
1
1
1
-
-
726132
Kwon
-
Biotic and abiotic stresses in ...
Atropa belladonna
Plant Biotechnol.
26
207-215
2009
-
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1
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1
1
1
1
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-
723402
Zubieta
Structural basis for substrate ...
Clarkia breweri
Plant Cell
15
1704-1716
2003
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1
1
5
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1
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1
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4
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1
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6
1
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5
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6
1
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1
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1
1
723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
2
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2
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4
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8
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10
2
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2
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6
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2
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2
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2
2
2
721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
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1
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1
6
2
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2
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4
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4
1
1
3
1
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1
1
3
1
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1
2
1
2
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-
721412
Ross
S-Adenosyl-L-methionine:salicy ...
Clarkia breweri
Arch. Biochem. Biophys.
367
9-16
1999
-
-
1
-
-
-
8
3
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1
3
1
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6
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1
1
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4
4
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5
1
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1
2
1
1
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1
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8
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3
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1
3
1
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4
4
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1
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2
1
1
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