BRENDA - Enzyme Database show
show all sequences of 2.1.1.274

Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode

Lin, J.; Mazarei, M.; Zhao, N.; Zhu, J.J.; Zhuang, X.; Liu, W.; Pantalone, V.R.; Arelli, P.R.; Stewart, C.N.; Chen, F.; Plant Biotechnol. J. 11, 1135-1145 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells
Glycine max
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
about 65% residual activity at 5 mM
Glycine max
Cu2+
complete inhibition at 5 mM
Glycine max
Fe2+
complete inhibition at 5 mM
Glycine max
Fe3+
complete inhibition at 5 mM
Glycine max
Mn2+
about 25% residual activity at 5 mM
Glycine max
Zn2+
complete inhibition at 5 mM
Glycine max
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00046
-
salicylate
in 50 mM Tris-HCl, at pH 7.5 and 25C
Glycine max
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
2fold increase in activity in the presence of 5 mM K+
Glycine max
additional information
Na+, NH4+ and Mg2+ have no effect on isoform SAMT1 activity
Glycine max
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Glycine max
jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1
?
-
-
-
S-adenosyl-L-methionine + 3-hydroxybenzoate
Glycine max
1.8% activity compared to salicylate
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
?
S-adenosyl-L-methionine + anthranilate
Glycine max
9.3% activity compared to salicylate
?
-
-
?
S-adenosyl-L-methionine + benzoate
Glycine max
16.9% activity compared to salicylate
?
-
-
?
S-adenosyl-L-methionine + salicylate
Glycine max
highest level of specific activity with salicylate
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hairy root
-
Glycine max
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1
726129
Glycine max
?
-
-
-
-
S-adenosyl-L-methionine + 3-hydroxybenzoate
1.8% activity compared to salicylate
726129
Glycine max
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
-
?
S-adenosyl-L-methionine + anthranilate
9.3% activity compared to salicylate
726129
Glycine max
?
-
-
-
?
S-adenosyl-L-methionine + benzoate
16.9% activity compared to salicylate
726129
Glycine max
?
-
-
-
?
S-adenosyl-L-methionine + salicylate
highest level of specific activity with salicylate
726129
Glycine max
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Glycine max
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
40
about 80% activity at 10C, about 90% activity at 20C, about 78% activity at 30C, about 70% activity at 40C
Glycine max
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Glycine max
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8
about 90% activity at pH 7.0, about 40% activity at pH 8.0
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells
Glycine max
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
about 65% residual activity at 5 mM
Glycine max
Cu2+
complete inhibition at 5 mM
Glycine max
Fe2+
complete inhibition at 5 mM
Glycine max
Fe3+
complete inhibition at 5 mM
Glycine max
Mn2+
about 25% residual activity at 5 mM
Glycine max
Zn2+
complete inhibition at 5 mM
Glycine max
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00046
-
salicylate
in 50 mM Tris-HCl, at pH 7.5 and 25C
Glycine max
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
2fold increase in activity in the presence of 5 mM K+
Glycine max
additional information
Na+, NH4+ and Mg2+ have no effect on isoform SAMT1 activity
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Glycine max
jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1
?
-
-
-
S-adenosyl-L-methionine + 3-hydroxybenzoate
Glycine max
1.8% activity compared to salicylate
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
?
S-adenosyl-L-methionine + anthranilate
Glycine max
9.3% activity compared to salicylate
?
-
-
?
S-adenosyl-L-methionine + benzoate
Glycine max
16.9% activity compared to salicylate
?
-
-
?
S-adenosyl-L-methionine + salicylate
Glycine max
highest level of specific activity with salicylate
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hairy root
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1
726129
Glycine max
?
-
-
-
-
S-adenosyl-L-methionine + 3-hydroxybenzoate
1.8% activity compared to salicylate
726129
Glycine max
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
-
?
S-adenosyl-L-methionine + anthranilate
9.3% activity compared to salicylate
726129
Glycine max
?
-
-
-
?
S-adenosyl-L-methionine + benzoate
16.9% activity compared to salicylate
726129
Glycine max
?
-
-
-
?
S-adenosyl-L-methionine + salicylate
highest level of specific activity with salicylate
726129
Glycine max
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Glycine max
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
40
about 80% activity at 10C, about 90% activity at 20C, about 78% activity at 30C, about 70% activity at 40C
Glycine max
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Glycine max
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8
about 90% activity at pH 7.0, about 40% activity at pH 8.0
Glycine max
General Information
General Information
Commentary
Organism
physiological function
isoform SAM1 plays a role in soybean defence against the soybean cyst nematode Heterodera glycines. Enzyme overexpression also affects the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction
Glycine max
General Information (protein specific)
General Information
Commentary
Organism
physiological function
isoform SAM1 plays a role in soybean defence against the soybean cyst nematode Heterodera glycines. Enzyme overexpression also affects the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction
Glycine max
Other publictions for EC 2.1.1.274
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736189
Wang
Cloning and characterization o ...
Lilium hybrid cultivar
Genet. Mol. Res.
14
14510-14521
2015
-
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
2
2
-
-
-
736696
Krol
Priming of seeds with methyl j ...
Solanum lycopersicum
J. Plant Physiol.
179
122-132
2015
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
736842
Ludwig-Mueller
A novel methyltransferase from ...
Plasmodiophora brassicae
Mol. Plant Pathol.
16
349-364
2015
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
736841
Zhu
Salicylic acid and jasmonic ac ...
Nicotiana benthamiana
Mol. Plant Microbe Interact.
27
567-577
2014
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726129
Lin
Overexpression of a soybean sa ...
Glycine max
Plant Biotechnol. J.
11
1135-1145
2013
-
-
1
-
-
-
6
1
-
2
-
5
-
5
-
-
-
-
-
1
-
-
5
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
1
-
2
-
5
-
-
-
-
-
1
-
-
5
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
725781
Yao
QM/MM free energy simulations ...
Clarkia breweri
J. Phys. Chem. B
115
389-396
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726013
Liu
Altering expression of benzoic ...
Arabidopsis thaliana
Mol. Plant Microbe Interact.
23
82-90
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726172
Tieman
Functional analysis of a tomat ...
Solanum lycopersicum
Plant J.
62
113-123
2010
-
-
1
-
-
-
-
2
-
-
-
4
-
5
-
-
-
-
-
4
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
2
-
-
-
-
1
1
1
1
-
-
726132
Kwon
-
Biotic and abiotic stresses in ...
Atropa belladonna
Plant Biotechnol.
26
207-215
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
723402
Zubieta
Structural basis for substrate ...
Clarkia breweri
Plant Cell
15
1704-1716
2003
-
-
1
1
5
-
-
1
-
-
1
-
-
4
-
-
1
-
-
-
-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
2
-
2
-
-
-
-
4
-
-
8
-
-
2
-
-
2
-
-
6
-
-
10
2
-
-
-
2
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
4
-
-
8
-
-
-
-
2
-
6
-
-
10
2
-
-
-
2
2
-
-
-
2
-
-
2
2
2
721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
-
-
1
-
-
1
6
2
-
-
2
-
-
4
-
-
1
-
-
4
1
1
3
1
-
-
1
2
1
2
-
-
-
-
-
-
-
1
-
-
-
1
-
6
-
2
-
-
2
-
-
-
-
1
-
4
1
1
3
1
-
-
1
2
1
2
-
-
-
-
-
-
-
-
721412
Ross
S-Adenosyl-L-methionine:salicy ...
Clarkia breweri
Arch. Biochem. Biophys.
367
9-16
1999
-
-
1
-
-
-
8
3
-
1
3
1
-
6
-
-
1
1
-
4
4
-
5
1
-
-
1
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
3
-
1
3
1
-
-
-
1
-
4
4
-
5
1
-
-
1
2
1
1
-
-
-
-
-
-
-
-