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Literature summary for 2.1.1.274 extracted from

  • Lin, J.; Mazarei, M.; Zhao, N.; Zhu, J.J.; Zhuang, X.; Liu, W.; Pantalone, V.R.; Arelli, P.R.; Stewart, C.N.; Chen, F.
    Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode (2013), Plant Biotechnol. J., 11, 1135-1145.
    View publication on PubMed
Search for more literature for 2.1.1.274

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 65% residual activity at 5 mM Glycine max
Cu2+ complete inhibition at 5 mM Glycine max
Fe2+ complete inhibition at 5 mM Glycine max
Fe3+ complete inhibition at 5 mM Glycine max
Mn2+ about 25% residual activity at 5 mM Glycine max
Zn2+ complete inhibition at 5 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00046
-
 salicylate in 50 mM Tris-HCl, at pH 7.5 and 25°C Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 2fold increase in activity in the presence of 5 mM K+ Glycine max
additional information Na+, NH4+ and Mg2+ have no effect on isoform SAMT1 activity Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 ?
-
 ?
S-adenosyl-L-methionine + 3-hydroxybenzoate Glycine max 1.8% activity compared to salicylate S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
 ?
S-adenosyl-L-methionine + anthranilate Glycine max 9.3% activity compared to salicylate ?
-
 ?
S-adenosyl-L-methionine + benzoate Glycine max 16.9% activity compared to salicylate ?
-
 ?
S-adenosyl-L-methionine + salicylate Glycine max highest level of specific activity with salicylate S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 Glycine max ?
-
 ?
S-adenosyl-L-methionine + 3-hydroxybenzoate 1.8% activity compared to salicylate Glycine max S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
 ?
S-adenosyl-L-methionine + anthranilate 9.3% activity compared to salicylate Glycine max ?
-
 ?
S-adenosyl-L-methionine + benzoate 16.9% activity compared to salicylate Glycine max ?
-
 ?
S-adenosyl-L-methionine + salicylate highest level of specific activity with salicylate Glycine max S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Synonyms

Synonyms Comment Organism
salicylic acid methyltransferase
-
 Glycine max
SAMT1
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 about 80% activity at 10°C, about 90% activity at 20°C, about 78% activity at 30°C, about 70% activity at 40°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7 8 about 90% activity at pH 7.0, about 40% activity at pH 8.0 Glycine max

General Information

General Information Comment Organism
physiological function isoform SAM1 plays a role in soybean defence against the soybean cyst nematode Heterodera glycines. Enzyme overexpression also affects the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction Glycine max