BRENDA - Enzyme Database
show all sequences of 2.1.1.274

Cloning and characterization of a benzoic acid/salicylic acid carboxyl methyltransferase gene involved in floral scent production from lily (Lilium Yelloween)

Wang, H.; Sun, M.; Li, L.L.; Xie, X.H.; Zhang, Q.X.; Genet. Mol. Res. 14, 14510-14521 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene LiBSMT, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis and tree, quantitative real-time PCR expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Lilium hybrid cultivar
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41050
-
x * 41050, about, sequence calculation
Lilium hybrid cultivar
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lilium hybrid cultivar
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
?
-
-
-
S-adenosyl-L-methionine + salicylate
Lilium hybrid cultivar
-
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lilium hybrid cultivar
A0A075W3C7
var. Yelloween, a popular OT (oriental x trumpet) hybrid, collected from the field in Xiaotang­shan, Beijing, gene LiBSMT
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Lilium hybrid cultivar
-
petal
-
Lilium hybrid cultivar
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
736189
Lilium hybrid cultivar
?
-
-
-
-
S-adenosyl-L-methionine + salicylate
-
736189
Lilium hybrid cultivar
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41050, about, sequence calculation
Lilium hybrid cultivar
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Lilium hybrid cultivar
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Lilium hybrid cultivar
sequence calculation
-
5.67
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LiBSMT, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis and tree, quantitative real-time PCR expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Lilium hybrid cultivar
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Lilium hybrid cultivar
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41050
-
x * 41050, about, sequence calculation
Lilium hybrid cultivar
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lilium hybrid cultivar
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
?
-
-
-
S-adenosyl-L-methionine + salicylate
Lilium hybrid cultivar
-
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Lilium hybrid cultivar
-
petal
-
Lilium hybrid cultivar
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273
736189
Lilium hybrid cultivar
?
-
-
-
-
S-adenosyl-L-methionine + salicylate
-
736189
Lilium hybrid cultivar
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41050, about, sequence calculation
Lilium hybrid cultivar
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Lilium hybrid cultivar
sequence calculation
-
5.67
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to SABATH family, a class of O-methyltransferases and N-methyltransferases
Lilium hybrid cultivar
physiological function
enzyme benzoic acid/salicylic acid carboxyl methyltransferase is enzyme responsible for catalyzing benzoic acid and salicylic acid to methyl benzoate and methyl salicylate, respectively, and is involved in floral scent production from lily
Lilium hybrid cultivar
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to SABATH family, a class of O-methyltransferases and N-methyltransferases
Lilium hybrid cultivar
physiological function
enzyme benzoic acid/salicylic acid carboxyl methyltransferase is enzyme responsible for catalyzing benzoic acid and salicylic acid to methyl benzoate and methyl salicylate, respectively, and is involved in floral scent production from lily
Lilium hybrid cultivar
Other publictions for EC 2.1.1.274
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736189
Wang
Cloning and characterization o ...
Lilium hybrid cultivar
Genet. Mol. Res.
14
14510-14521
2015
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736696
Krol
Priming of seeds with methyl j ...
Solanum lycopersicum
J. Plant Physiol.
179
122-132
2015
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736842
Ludwig-Mueller
A novel methyltransferase from ...
Plasmodiophora brassicae
Mol. Plant Pathol.
16
349-364
2015
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736841
Zhu
Salicylic acid and jasmonic ac ...
Nicotiana benthamiana
Mol. Plant Microbe Interact.
27
567-577
2014
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726129
Lin
Overexpression of a soybean sa ...
Glycine max
Plant Biotechnol. J.
11
1135-1145
2013
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725781
Yao
QM/MM free energy simulations ...
Clarkia breweri
J. Phys. Chem. B
115
389-396
2011
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726013
Liu
Altering expression of benzoic ...
Arabidopsis thaliana
Mol. Plant Microbe Interact.
23
82-90
2010
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2
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726172
Tieman
Functional analysis of a tomat ...
Solanum lycopersicum
Plant J.
62
113-123
2010
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726132
Kwon
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Biotic and abiotic stresses in ...
Atropa belladonna
Plant Biotechnol.
26
207-215
2009
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723402
Zubieta
Structural basis for substrate ...
Clarkia breweri
Plant Cell
15
1704-1716
2003
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723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
2
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2
721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
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721412
Ross
S-Adenosyl-L-methionine:salicy ...
Clarkia breweri
Arch. Biochem. Biophys.
367
9-16
1999
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1
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8
3
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1
3
1
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6
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1
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4
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1
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8
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