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Literature summary for 2.1.1.273 extracted from

  • Ludwig-Mueller, J.; Juelke, S.; Geiss, K.; Richter, F.; Mithoefer, A.; ?ola, I.; Rusak, G.; Keenan, S.; Bulman, S.
    A novel methyltransferase from the intracellular pathogen Plasmodiophora brassicae methylates salicylic acid (2015), Mol. Plant Pathol., 16, 349-364.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus Plasmodiophora brassicae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Plasmodiophora brassicae the enzyme also catalyzes the reaction of salicylate 1-O-methyltransferase, EC 2.1.1.274 ?
-
 ?
S-adenosyl-L-methionine + benzoate Plasmodiophora brassicae
-
 S-adenosyl-L-homocysteine + methyl benzoate
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodiophora brassicae R4I7S9 gene PbBSMT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography Plasmodiophora brassicae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0072
-
 purified recombinant enzyme, pH and temperature not specified in the publication Plasmodiophora brassicae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also catalyzes the reaction of salicylate 1-O-methyltransferase, EC 2.1.1.274 Plasmodiophora brassicae ?
-
 ?
S-adenosyl-L-methionine + benzoate
-
 Plasmodiophora brassicae S-adenosyl-L-homocysteine + methyl benzoate
-
 ?

Synonyms

Synonyms Comment Organism
benzoic acid/salicylic acid methyltransferase
-
 Plasmodiophora brassicae
BSMT
-
 Plasmodiophora brassicae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Plasmodiophora brassicae

General Information

General Information Comment Organism
physiological function the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate Plasmodiophora brassicae