Cloned (Comment) | Organism |
---|---|
gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus | Plasmodiophora brassicae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodiophora brassicae | the enzyme also catalyzes the reaction of salicylate 1-O-methyltransferase, EC 2.1.1.274 | ? | - |
? | |
S-adenosyl-L-methionine + benzoate | Plasmodiophora brassicae | - |
S-adenosyl-L-homocysteine + methyl benzoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodiophora brassicae | R4I7S9 | gene PbBSMT | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography | Plasmodiophora brassicae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0072 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Plasmodiophora brassicae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also catalyzes the reaction of salicylate 1-O-methyltransferase, EC 2.1.1.274 | Plasmodiophora brassicae | ? | - |
? | |
S-adenosyl-L-methionine + benzoate | - |
Plasmodiophora brassicae | S-adenosyl-L-homocysteine + methyl benzoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
benzoic acid/salicylic acid methyltransferase | - |
Plasmodiophora brassicae |
BSMT | - |
Plasmodiophora brassicae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Plasmodiophora brassicae |
General Information | Comment | Organism |
---|---|---|
physiological function | the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate | Plasmodiophora brassicae |