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Literature summary for 2.1.1.273 extracted from

  • Dudareva, N.; Murfitt, L.M.; Mann, C.J.; Gorenstein, N.; Kolosova, N.; Kish, C.M.; Bonham, C.; Wood, K.
    Developmental regulation of methyl benzoate biosynthesis and emission in snapdragon flowers (2000), Plant Cell, 12, 949-961.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.273

Cloned(Commentary)

Cloned (Comment) Organism
BAMT cDNA expressed in Escherichia coli Clarkia breweri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + benzoate Clarkia breweri emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity S-adenosyl-L-homocysteine + methyl benzoate
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 ?

Organism

Organism UniProt Comment Textmining
Clarkia breweri
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-
-

Purification (Commentary)

Purification (Comment) Organism
from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenyl–Sepharose 6FF and MonoQ chromatography Clarkia breweri

Source Tissue

Source Tissue Comment Organism Textmining
anther reduced BAMT activity Clarkia breweri
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corolla tube reduced BAMT activity Clarkia breweri
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additional information no BAMT activity in pistil, stamen, sepal, ovary and leaf tissue Clarkia breweri
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petal in upper and lower lobes, major activity of BAMT Clarkia breweri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 emission of methyl benzoate begins at anthesis but at a very low level, 1.8 mg per flower for 24 hr, reaches a peak between days 5 to 8, and declines thereafter, at peak, 56.5 mg is emitted per flower in 24 hr Clarkia breweri
0.0004518
-
 recombinant protein, with benzoic acid as substrate, pH 6.9, 20°C Clarkia breweri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity detected with salicylic acid and trans-cinnamic acid and their derivatives 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, benzyl alcohol, and 2-,3- and 4-coumaric acid Clarkia breweri ?
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 ?
S-adenosyl-L-methionine + benzoate emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity Clarkia breweri S-adenosyl-L-homocysteine + methyl benzoate
-
 ?
S-adenosyl-L-methionine + benzoate methyl benzoate is the most abundant scent compound detected in the majority of snapdragon varieties Clarkia breweri S-adenosyl-L-homocysteine + methyl benzoate
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 ?

Synonyms

Synonyms Comment Organism
BAMT
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 Clarkia breweri
S-adenosyl-L-methionine:benzoic acid carboxyl methyl transferase
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 Clarkia breweri

Expression

Organism Comment Expression
Clarkia breweri during flower life span, levels of methyl benzoate emission, BAMT activity, BAMT gene expression, and the amounts of BAMT protein and benzoic acid are developmentally and differentially regulated additional information

General Information

General Information Comment Organism
metabolism final enzyme in the biosynthesis of methyl benzoate Clarkia breweri
physiological function attracting pollinators like bumblebees Clarkia breweri