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Literature summary for 2.1.1.270 extracted from

  • Liu, C.J.; Deavours, B.E.; Richard, S.B.; Ferrer, J.L.
    Blount, J.W.; Huhman, D.; Dixon, R.A.; Noel, J.P.: Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses (2006), Plant Cell, 18, 3656-3669.
    View publication on PubMed
Search for more literature for 2.1.1.270

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Medicago truncatula

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized from a polyethylene glycol precipitant containing 2.5 mM of the reaction product S-adenosyl-L-homocysteine, 2.5 A resolution Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
 (+)-6a-hydroxymaackiain pH 8.0, 30°C Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Medicago truncatula
-
 S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago truncatula Q29U70
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
-
 Medicago truncatula S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain the bifunctional enzyme also catalyzes the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanonewith slightly higher catalytic efficiency Medicago truncatula S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Synonyms

Synonyms Comment Organism
HI4'OMT/HM3OMT bifunctional enzyme Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Medicago truncatula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Medicago truncatula

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
197
-
 (+)-6a-hydroxymaackiain pH 8.0, 30°C Medicago truncatula