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Literature summary for 2.1.1.270 extracted from

  • Akashi, T.; VanEtten, H.D.; Sawada, Y.; Wasmann, C.C.; Uchiyama, H.; Ayabe, S.
    Catalytic specificity of pea O-methyltransferases suggests gene duplication for (+)-pisatin biosynthesis (2006), Phytochemistry, 67, 2525-2530.
    View publication on PubMed
Search for more literature for 2.1.1.270

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pisum sativum

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of the active sites of both HMM1 and HMM2 with substrate (+)-6a-hydroxymaackiain Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 (+)-6a-hydroxymaackiain pH 7.5, 30°C Pisum sativum
0.0005
-
 (+)-6a-hydroxymaackiain isoform HMM2, 30°C Pisum sativum
0.006
-
 (+)-6a-hydroxymaackiain isoform HMM1, 30°C Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Pisum sativum HMM2 is involved primarily in the biosynthesis of (+)-pisatin S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain isoform HMM1 shows efficiencies for the methylation of 2,7,4'-trihydroxyisoflavanone 4times higher than for the methylation of (+)-6a-hydroxymaackiain, substrate of 6a-hydroxymaackiain-3-O-methyltransferase. Isoform HMM2 has a higher Vmax and lower Km on (+)-6a-hydroxymaackiain, and has a 67fold higher efficiency for the methylation of (+)-6a-hydroxymaackiain than that for 2,7,4'-trihydroxyisoflavanone Pisum sativum S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain HMM2 is involved primarily in the biosynthesis of (+)-pisatin Pisum sativum S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain HMM2 shows efficiencies (relative Vmax/Km) for the 3-O-methylation of (+)-6a-hydroxymaackiain 67 times higher than for the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanone Pisum sativum S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Synonyms

Synonyms Comment Organism
HMM1
-
 Pisum sativum
HMM2
-
 Pisum sativum
S-adenosyl-L-methionine: 2-hydroxyisoflavanone 4'-O-methyltransferase
-
 Pisum sativum