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Literature summary for 2.1.1.270 extracted from

  • Akashi, T.; VanEtten, H.D.; Sawada, Y.; Wasmann, C.C.; Uchiyama, H.; Ayabe, S.
    Catalytic specificity of pea O-methyltransferases suggests gene duplication for (+)-pisatin biosynthesis (2006), Phytochemistry, 67, 2525-2530.
    View publication on PubMed
Search for more literature for 2.1.1.270

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lathyrus oleraceus

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of the active sites of both HMM1 and HMM2 with substrate (+)-6a-hydroxymaackiain Lathyrus oleraceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 (+)-6a-hydroxymaackiain isoform HMM2, 30°C Lathyrus oleraceus
0.0005
-
 (+)-6a-hydroxymaackiain pH 7.5, 30°C Lathyrus oleraceus
0.006
-
 (+)-6a-hydroxymaackiain isoform HMM1, 30°C Lathyrus oleraceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Lathyrus oleraceus HMM2 is involved primarily in the biosynthesis of (+)-pisatin S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Organism

Organism UniProt Comment Textmining
Lathyrus oleraceus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain isoform HMM1 shows efficiencies for the methylation of 2,7,4'-trihydroxyisoflavanone 4times higher than for the methylation of (+)-6a-hydroxymaackiain, substrate of 6a-hydroxymaackiain-3-O-methyltransferase. Isoform HMM2 has a higher Vmax and lower Km on (+)-6a-hydroxymaackiain, and has a 67fold higher efficiency for the methylation of (+)-6a-hydroxymaackiain than that for 2,7,4'-trihydroxyisoflavanone Lathyrus oleraceus S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain HMM2 is involved primarily in the biosynthesis of (+)-pisatin Lathyrus oleraceus S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain HMM2 shows efficiencies (relative Vmax/Km) for the 3-O-methylation of (+)-6a-hydroxymaackiain 67 times higher than for the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanone Lathyrus oleraceus S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Synonyms

Synonyms Comment Organism
HMM1
-
 Lathyrus oleraceus
HMM2
-
 Lathyrus oleraceus
S-adenosyl-L-methionine: 2-hydroxyisoflavanone 4'-O-methyltransferase
-
 Lathyrus oleraceus