Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.268 extracted from

  • Huang, M.H.; Wang, J.T.; Zhang, J.H.; Mao, X.L.; Peng, G.X.; Lin, X.; Lv, D.; Yuan, C.; Lin, H.; Wang, E.D.; Zhou, X.L.
    Mitochondrial RNA m3C methyltransferase METTL8 relies on an isoform-specific N-terminal extension and modifies multiple heterogenous tRNAs (2023), Sci. Bull. (Beijing), 68, 2094-2105.
    View publication on PubMed
Search for more literature for 2.1.1.268

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D38A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
D49A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
H45A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
K41A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
N46A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
R34A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
S33A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
T37A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information t6A37 inhibits N3-methylcytosine modification by the enzyme at position 32 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine32 in tRNASer Homo sapiens
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNAThr Homo sapiens
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isoform METTL8-Iso4 is inactive in N3-methylcytosine32 generation due to the lack of N-extension Homo sapiens ?
-
-
S-adenosyl-L-methionine + cytosine32 in tRNASer
-
 Homo sapiens S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNAThr
-
 Homo sapiens S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?

Synonyms

Synonyms Comment Organism
METTL8-Iso1 isoform Homo sapiens
METTL8-Iso4 inactive isoform Homo sapiens
RNA m3C32 methyltransferase
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function isoform METTL8-Iso1 substantially stimulates the aminoacylation activities of SARS2 and TARS2 in vitro, suggesting a functional connection between mitochondrial tRNA modification and charging Homo sapiens