Cloned (Comment) | Organism |
---|---|
gene yhiR, recombinant expresssion of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine2030 in 23S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P37634 | MG1655, gene yhiR | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine2030 in 23S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + adenine2030 in 23S rRNA | enzyme RlmJ uses the cofactor S-adenosyl-L-methionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli | Escherichia coli | S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
23S rRNA methyltransferase | - |
Escherichia coli |
ribosomal RNA large subunit methyltransferase J | UniProt | Escherichia coli |
rlmJ | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Escherichia coli |