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Literature summary for 2.1.1.266 extracted from

  • Golovina, A.Y.; Dzama, M.M.; Osterman, I.A.; Sergiev, P.V.; Serebryakova, M.V.; Bogdanov, A.A.; Dontsova, O.A.
    The last rRNA methyltransferase of E. coli revealed: The yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA (2012), RNA, 18, 1725-1734.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.266

Activating Compound

Activating Compound Comment Organism Structure
additional information YhiR by itself, without any additional proteins, is able to modify 23S rRNA Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene yhiR, expression as C-terminally His6-tagged protein in escherichia coli strain AG1 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information strain JW3466 lacks the yhiR gene on the chromosome, i.e. strain DELTAyhiR, phenotype of the yhiR gene knockout, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine2030 in 23S rRNA Escherichia coli nascent 23S rRNA is the natural substrate for YhiR S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Escherichia coli P37634 gene yhiR or rlmJ
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme activity with in vitro modified potential YhiR substrates prepared from the enzyme-deficient DELTAyhiR strain: methylation of the 50S subunits, NH4Cl/ethanol split particles, LiCl split particles, and deproteinized 23S rRNA, overview Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + adenine2030 in 23S rRNA nascent 23S rRNA is the natural substrate for YhiR Escherichia coli S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + adenine2030 in 23S rRNA adenine-N6 methyltransferase, encoded by gene yhiR, is specific for modification of A2030 of 23S ribosomal RNA. YhiR by itself, without any additional proteins, is able to modify 23S rRNA Escherichia coli S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA mass spectrometric product identification, overview ?

Synonyms

Synonyms Comment Organism
adenine-N6 methyltransferase
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 Escherichia coli
m6A2030 methyltransferase
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 Escherichia coli
rlmJ
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 Escherichia coli
YhiR
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 Escherichia coli
YhiR protein
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction the phenotype of yhiR knockout gene is very mild under various growth conditions and at the stationary phase, except for a small growth advantage at anaerobic conditions. A very small subset of genes is affected by yhiR inactivation, while the majority of the proteome remains independent of the A2030 modification. Lack of modification at the 23S rRNA nucleotide: A2030 does not cause the accumulation of assembly intermediates. Phenotype of the yhiR gene knockout, overview Escherichia coli
additional information the yhiR gene should be renamed to rlmJ according to the rRNA methyltransferase nomenclature Escherichia coli
physiological function the yhiR gene of Escherichia coli is solely responsible for the N6-methylation of A2030 of the 23S rRNA. Nucleotide m6A2030, buried inside the large ribosomal subunit close to the peptidyltransferase center, forms a strong stacked contact with the U571 residue of the 23S rRNA, thus connecting structural elements of domains II and V located at half-23S rRNA length distance in the primary structure. Modification of the 23S rRNA nucleotide A2030 occurs early in the 50S subunit assembly Escherichia coli