Cloned (Comment) | Organism |
---|---|
gene tehB, expression of His6-tagged wild-type enzyme in Escherichia coli strain coli C41(DE3), expression of the mutant TehB enzymes in tehAB operon from pTWT101, a pTZ19R clone, in Escherichia coli strain JM109, expression of His6-tagged mutant enzymes in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D36A | site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
D36N | site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
D59A | site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
D97E | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is unaltered | Escherichia coli |
D97N | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is partially reduced | Escherichia coli |
F98A | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
F98Y | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
additional information | mutagenesis by the two-stage PCR-based overlapping extension method | Escherichia coli |
Y96A | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is partially reduced | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26256 | - |
x * 26256, His6-tagged TehB, sequence caculation, x * 27000-28000, recombinant His6-tagged TehB, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tellurite | Escherichia coli | - |
S-adenosyl-L-homocysteine + methanetelluronate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25397 | gene tehB | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type TehB from Escherichia coli strains C41(DE3) and BL21(DE3), respectively, by nickel affinity chromatographhy | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tellurite | - |
Escherichia coli | S-adenosyl-L-homocysteine + methanetelluronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | x * 26256, His6-tagged TehB, sequence caculation, x * 27000-28000, recombinant His6-tagged TehB, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TehB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on, the conserved motifs I and II in TehB are involved in S-adenosyl-L-methionine binding | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | replacement of the conserved aspartate residue in motif I by asparagine or alanine, or of the conserved phenylalanine in motif II by tyrosine or alanine, decreases resistance to background levels | Escherichia coli |
metabolism | the Escherichia coli chromosomal determinant for tellurite resistance consists of two genes tehA and tehB, which, when expressed on a multicopy plasmid, confer resistance to K2TeO3 at 0.128 mg/ml | Escherichia coli |
physiological function | TehB utilizes a methyltransferase activity in the detoxification of tellurite | Escherichia coli |