Literature summary for 2.1.1.258 extracted from

Doukov, T.; Seravalli, J.; Stezowski, J.; Ragsdale, S.
Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase (2000), Structure, 8, 817-830.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834 cells	Moorella thermoacetica

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 9-15% (w/v) polyethylene glycol monomethyl ester 5000, 20-50 mM CaCl2, 20% (v/v) glycerol, and 50 mM HEPES, pH 7.5	Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	2 * 28000	Moorella thermoacetica

Organism

Organism	UniProt	Comment	Textmining
Moorella thermoacetica	Q46389	formerly Clostridium thermoaceticum	-

Purification (Commentary)

Purification (Comment)	Organism
phenyl Sepharose column chromatography and Q-Sepharose column chromatography	Moorella thermoacetica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate	-	Moorella thermoacetica	[methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 28000	Moorella thermoacetica

Synonyms

Synonyms	Comment	Organism
methyltetrahydrofolate, corrinoid ironsulfur protein methyltransferase	-	Moorella thermoacetica
MeTr	-	Moorella thermoacetica

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes a key step in the Wood-Ljungdahl pathway of carbon dioxide fixation	Moorella thermoacetica

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Release 2023.1 (January 2023)