Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
gene trmY or HVO_1989 | Haloferax volcanii |
gene VNG1980C, expression of the gene encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain | Halobacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of the gene VNG1980C encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain | Haloferax volcanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Halobacterium sp. | |
S-adenosyl-L-homocysteine | - |
Haloferax volcanii | |
S-adenosyl-L-homocysteine | - |
Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + pseudouridine54 in tRNA | Haloferax volcanii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | Halobacterium sp. | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | Halobacterium sp. NRC-1 | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium sp. | Q9HNR6 | gene VNG1980C | - |
Halobacterium sp. NRC-1 | Q9HNR6 | gene VNG1980C | - |
Haloferax volcanii | D4GTL8 | gene HVO_1989 or trmY | - |
Methanocaldococcus jannaschii | Q59034 | gene Mj1640 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Haloferax volcanii | ? | - |
? | |
additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Halobacterium sp. | ? | - |
? | |
additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Halobacterium sp. NRC-1 | ? | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in elongator tRNAMet | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in elongator tRNAMet | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Halobacterium sp. | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, in vitro and in vivo reaction, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Halobacterium sp. | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Halobacterium sp. NRC-1 | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Halobacterium sp. NRC-1 | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNATrp | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNATrp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COG1901 | - |
Haloferax volcanii |
COG1901 | - |
Halobacterium sp. |
COG1901 | - |
Methanocaldococcus jannaschii |
DUF358 | - |
Haloferax volcanii |
DUF358 | - |
Halobacterium sp. |
DUF358 | - |
Methanocaldococcus jannaschii |
SAM-dependent N1-pseudouridine methyltransferase | - |
Methanocaldococcus jannaschii |
TrmY protein | - |
Haloferax volcanii |
TrmY protein | - |
Halobacterium sp. |
TrmY protein | - |
Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Haloferax volcanii |
evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Halobacterium sp. |
evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Methanocaldococcus jannaschii |
malfunction | deletion of trmY leads to the absence of m1PSI54 in Haloferax volcanii tRNA | Haloferax volcanii |
metabolism | Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview | Haloferax volcanii |
metabolism | Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview | Methanocaldococcus jannaschii |
additional information | Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 | Methanocaldococcus jannaschii |
physiological function | trmY is not essential in Haloferax volcanii | Haloferax volcanii |