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Literature summary for 2.1.1.256 extracted from

  • Hirata, A.; Suzuki, T.; Nagano, T.; Fujii, D.; Okamoto, M.; Sora, M.; Lowe, T.M.; Kanai, T.; Atomi, H.; Suzuki, T.; Hori, H.
    Distinct modified nucleosides in tRNATrp from thehyperthermophilic archaeon Thermococcus kodakarensis and requirement of tRNA m2G10/m22G10 methyltransferase (archaeal Trm11) for survival at high temperatures (2019), J. Bacteriol., 201, e00448-19 .
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information construction of a trm11 trm14 double disruptant strain that shows a lack of 2-methylguanosine (m2G) at position 67 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine67 in tRNATrp Thermococcus kodakarensis
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 S-adenosyl-L-homocysteine + N2-methylguanine67 in tRNATrp
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information hyperthermophilic archaeon Thermococcus kodakarensis grows at 60-100°C Thermococcus kodakarensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine37 in tRNATrp
-
 ?
S-adenosyl-L-methionine + guanine54 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine54 in tRNATrp
-
 ?
S-adenosyl-L-methionine + guanine67 in tRNATrp
-
 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine67 in tRNATrp
-
 ?
S-adenosyl-L-methionine + guanine8 in tRNATrp
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 Thermococcus kodakarensis S-adenosyl-L-homocysteine + N2-methylguanine8 in tRNATrp
-
 ?

Synonyms

Synonyms Comment Organism
m2G6 methyltransferase
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 Thermococcus kodakarensis
Trm14
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 Thermococcus kodakarensis
tRNA m2G6 methyltransferase
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 Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Thermococcus kodakarensis

General Information

General Information Comment Organism
evolution a distinct tRNA 2'-O-methyltransferase, which methylates the 2'-OH of ribose at position 6 in tRNA and does not differentiate between adenine and cytosine, may exist in Thermococcus and Pyrococcus genera. In terms of the other enzymes responsible for the observed 2'-O-methylations, Cm56 is a product of Trm56 (EC 2.1.1.206) Thermococcus kodakarensis
malfunction the lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggests that this methylation is mediated by m2G6 methyltransferase Trm14 Thermococcus kodakarensis
physiological function the trm14 gene is responsible for the m2G67 modification. The modification site (G67) forms a Watson-Crick base pair with C6 in tRNA. Archaeal Trm14 methylates G6 in tRNA and contains a THUMP domain, which often recognizes the CCA terminus in tRNA. But it has not been confirmed that Thermococcus kodakarensis Trm14 methylates G6 in tRNA like Methanocaldococcus jannaschii Trm14. Furthermore, the presence of m2G6 modification in Thermococcus kodakarensis tRNAs is not confirmed. In total, trm14 is responsible for m2G at positions 8, 54, 37, and 67, respectively, as determined by analysis of tRNATrp from gene disruptant strains Thermococcus kodakarensis