BRENDA - Enzyme Database show
show all sequences of 2.1.1.256

The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N 2-methylguanosine at position 6 in tRNA

Roovers, M.; Oudjama, Y.; Fislage, M.; Bujnicki, J.; Versées, W.; Droogmans, L.; RNA 18, 815-824 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli Rosetta (DE3)
Pyrococcus furiosus
expression in Escherichia coli Rosetta (DE3)
Thermus thermophilus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
Q8U248
-
-
Thermus thermophilus
Q72IH5
-
-
Thermus thermophilus DSM 7039
Q72IH5
-
-
Purification (Commentary)
Commentary
Organism
-
Pyrococcus furiosus
-
Thermus thermophilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine6 in tRNA(Phe)
production of a small amount of N2-dimethylguanine6 in tRNA(Phe)
746421
Thermus thermophilus
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNA(Phe)
production of a small amount of N2-dimethylguanine6 in tRNA(Phe)
746421
Pyrococcus furiosus
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNA(Phe)
production of a small amount of N2-dimethylguanine6 in tRNA(Phe)
746421
Thermus thermophilus DSM 7039
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 39143, calculated from sequence
Thermus thermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli Rosetta (DE3)
Pyrococcus furiosus
expression in Escherichia coli Rosetta (DE3)
Thermus thermophilus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrococcus furiosus
-
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine6 in tRNA(Phe)
production of a small amount of N2-dimethylguanine6 in tRNA(Phe)
746421
Thermus thermophilus
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNA(Phe)
production of a small amount of N2-dimethylguanine6 in tRNA(Phe)
746421
Pyrococcus furiosus
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNA(Phe)
production of a small amount of N2-dimethylguanine6 in tRNA(Phe)
746421
Thermus thermophilus DSM 7039
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 39143, calculated from sequence
Thermus thermophilus
General Information
General Information
Commentary
Organism
malfunction
inactivation of the trmN gene leads to a total absence of N2-methylguanine in tRNA but did not affect cell growth or the formation of other modified nucleosides in tRNA(Phe). Therefore, m2G6 does not appear to be involved in an essential function
Thermus thermophilus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
inactivation of the trmN gene leads to a total absence of N2-methylguanine in tRNA but did not affect cell growth or the formation of other modified nucleosides in tRNA(Phe). Therefore, m2G6 does not appear to be involved in an essential function
Thermus thermophilus
Other publictions for EC 2.1.1.256
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720602
Fislage
Crystal structures of the tRNA ...
Pyrococcus furiosus, Thermus thermophilus
Nucleic Acids Res.
40
5149-5161
2012
-
-
2
2
-
-
2
-
-
2
-
2
-
7
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
2
-
2
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
2
2
-
-
-
746421
Roovers
The open reading frame TTC115 ...
Pyrococcus furiosus, Thermus thermophilus, Thermus thermophilus DSM 7039
RNA
18
815-824
2012
-
-
2
-
-
-
-
-
-
-
-
-
-
6
-
-
2
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720590
Menezes
Formation of m2G6 in Methanoca ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii JAL-1
Nucleic Acids Res.
39
7641-7655
2011
-
-
1
-
-
-
-
1
-
1
-
2
-
4
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-