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Literature summary for 2.1.1.256 extracted from

  • Roovers, M.; Oudjama, Y.; Fislage, M.; Bujnicki, J.; Vers้es, W.; Droogmans, L.
    The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N 2-methylguanosine at position 6 in tRNA (2012), RNA, 18, 815-824 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.256

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta (DE3) Thermus thermophilus
expression in Escherichia coli Rosetta (DE3) Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U248
-
-
Thermus thermophilus Q72IH5
-
-
Thermus thermophilus DSM 7039 Q72IH5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine6 in tRNA(Phe) production of a small amount of N2-dimethylguanine6 in tRNA(Phe) Thermus thermophilus S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
 ?
S-adenosyl-L-methionine + guanine6 in tRNA(Phe) production of a small amount of N2-dimethylguanine6 in tRNA(Phe) Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
 ?
S-adenosyl-L-methionine + guanine6 in tRNA(Phe) production of a small amount of N2-dimethylguanine6 in tRNA(Phe) Thermus thermophilus DSM 7039 S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39143, calculated from sequence Thermus thermophilus

Synonyms

Synonyms Comment Organism
PF1002
-
 Pyrococcus furiosus
TrmN
-
 Thermus thermophilus
tRNA (m2G6) methyltransferase
-
 Thermus thermophilus
tRNA (m2G6) methyltransferase
-
 Pyrococcus furiosus
TTC1157
-
 Thermus thermophilus

General Information

General Information Comment Organism
malfunction inactivation of the trmN gene leads to a total absence of N2-methylguanine in tRNA but did not affect cell growth or the formation of other modified nucleosides in tRNA(Phe). Therefore, m2G6 does not appear to be involved in an essential function Thermus thermophilus