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Literature summary for 2.1.1.255 extracted from

  • Ariyawutthiphan, O.; Ose, T.; Tsuda, M.; Gao, Y.; Yao, M.; Minami, A.; Oikawa, H.; Tanaka, I.
    Crystallization and preliminary X-ray crystallographic study of a methyltransferase involved in 2-methylisoborneol biosynthesis in Streptomyces lasaliensis (2011), Acta Crystallogr. Sect. F, 67, 417-420.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Streptomyces lasalocidi
overproduced in Escherichia coli strain BL21 Streptomyces lasalocidi

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged apo-GPPMT containing one molecule in the asymmetric unit, or in ternary complex with geranyl diphosphate and sinefungin, hanging drop method, mixing of protein solution containing 5 mg/ml in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl and 10% glycerol, with S-adenosyl-L-methionine at a protein:S-adenosyl-L-methionine molar ratio of 1:7, 4°C, and further with reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 1.4 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form I, or 50 mM sodium cacodylate, pH 6.7, 1.3 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form II, method screening, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolution, molecular replacement method Streptomyces lasalocidi
sitting-drop vapour-diffusion method Streptomyces lasalocidi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + geranyl diphosphate Streptomyces lasalocidi methylation of geranyl diphosphate before the cyclization reaction S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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?

Organism

Organism UniProt Comment Textmining
Streptomyces lasalocidi D3KYU3
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-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces lasalocidi
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration Streptomyces lasalocidi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + geranyl diphosphate methylation of geranyl diphosphate before the cyclization reaction Streptomyces lasalocidi S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
geranyl pyrophosphate methyltransferase
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Streptomyces lasalocidi
GPP methyltransferase
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Streptomyces lasalocidi
GPPMT
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Streptomyces lasalocidi

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Streptomyces lasalocidi