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show all sequences of 2.1.1.25

Purification and properties of an S-adenosylmethionine 2,4-disubstituted phenol O-methyltransferase from Phanerochaete chrysosporium

Coulter, C.; Kennedy, J.T.; McRoberts, W.C.; Harper, D.B.; Appl. Environ. Microbiol. 59, 706-711 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
0.1 mM, no resiudal activity
Phanerochaete chrysosporium
Cu2+
0.1 mM, no resiudal activity
Phanerochaete chrysosporium
Hg2+
0.1 mM, no resiudal activity
Phanerochaete chrysosporium
additional information
not inhibitory: EDTA, Mg2+, Ca2+, iodoacetamide
Phanerochaete chrysosporium
Phenylmercuriacetate
0.01 mM, no resiudal activity
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine
product inhibition
Phanerochaete chrysosporium
Zn2+
0.1 mM, 45% resiudal activity
Phanerochaete chrysosporium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
4-hydroxy-3-methoxyacetophenone
pH 8.0, 37C
Phanerochaete chrysosporium
0.099
-
S-adenosyl-L-methionine
pH 8.0, 37C
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Phanerochaete chrysosporium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
-
-
-
Purification (Commentary)
Commentary
Organism
-
Phanerochaete chrysosporium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
101
-
pH 8.0, 37C
Phanerochaete chrysosporium
Storage Stability
Storage Stability
Organism
-4C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, 75% loss of activity, in presence of 10% glycerol 5% loss of activtiy
Phanerochaete chrysosporium
2C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, a half-life of approximately 30 h
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
3-methoxy- and 3,5-dimethoxy-substituted 4-hydroxy-benzaldehydes, -benzoic acids, and -acetophenones are the preferred substrates. The corresponding 3,4-dihydroxy compounds are methylated relatively slowly, while the 3-hydroxy-4-methoxy compounds are almost inactive as substrates. Substituents in both the 2 and 4 positions relative to the hydroxyl group appear to be essential for significant enzyme attack of a substrate. Xenobiotic compounds such as 2,4-dichlorophenol and 2,4-dibromophenol are methylated almost as readily as 4-hydroxy-3-methoxyacetophenone. No substrates: homovanillic acid, caffeic acid, ferulic acid
741668
Phanerochaete chrysosporium
?
-
-
-
-
S-adenosyl-L-methionine + 2,4-dibromophenol
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 2,4-dibromo-1-methoxybenzene
-
-
-
?
S-adenosyl-L-methionine + 2,4-dichlorophenol
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 2,4-dichloro-1-methoxybenzene
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxyacetophenone
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4,5-trimethoxyacetophenone
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzaldehyde
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzaldehyde
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzoic acid
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzoic acid
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3-methoxyacetophenone
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4-dimethoxyacetophenone
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzaldehyde
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4-dimethoxybenzaldehyde
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzoic acid
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4-dimethoxybenzoic acid
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 54000, SDS-PAGE
Phanerochaete chrysosporium
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
rapid inactivation above
Phanerochaete chrysosporium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
-
Phanerochaete chrysosporium
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.041
-
S-adenosyl-L-homocysteine
pH 8.0, 37C
Phanerochaete chrysosporium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
0.1 mM, no resiudal activity
Phanerochaete chrysosporium
Cu2+
0.1 mM, no resiudal activity
Phanerochaete chrysosporium
Hg2+
0.1 mM, no resiudal activity
Phanerochaete chrysosporium
additional information
not inhibitory: EDTA, Mg2+, Ca2+, iodoacetamide
Phanerochaete chrysosporium
Phenylmercuriacetate
0.01 mM, no resiudal activity
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine
product inhibition
Phanerochaete chrysosporium
Zn2+
0.1 mM, 45% resiudal activity
Phanerochaete chrysosporium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.041
-
S-adenosyl-L-homocysteine
pH 8.0, 37C
Phanerochaete chrysosporium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
4-hydroxy-3-methoxyacetophenone
pH 8.0, 37C
Phanerochaete chrysosporium
0.099
-
S-adenosyl-L-methionine
pH 8.0, 37C
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Phanerochaete chrysosporium
Purification (Commentary) (protein specific)
Commentary
Organism
-
Phanerochaete chrysosporium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
101
-
pH 8.0, 37C
Phanerochaete chrysosporium
Storage Stability (protein specific)
Storage Stability
Organism
-4C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, 75% loss of activity, in presence of 10% glycerol 5% loss of activtiy
Phanerochaete chrysosporium
2C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, a half-life of approximately 30 h
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
3-methoxy- and 3,5-dimethoxy-substituted 4-hydroxy-benzaldehydes, -benzoic acids, and -acetophenones are the preferred substrates. The corresponding 3,4-dihydroxy compounds are methylated relatively slowly, while the 3-hydroxy-4-methoxy compounds are almost inactive as substrates. Substituents in both the 2 and 4 positions relative to the hydroxyl group appear to be essential for significant enzyme attack of a substrate. Xenobiotic compounds such as 2,4-dichlorophenol and 2,4-dibromophenol are methylated almost as readily as 4-hydroxy-3-methoxyacetophenone. No substrates: homovanillic acid, caffeic acid, ferulic acid
741668
Phanerochaete chrysosporium
?
-
-
-
-
S-adenosyl-L-methionine + 2,4-dibromophenol
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 2,4-dibromo-1-methoxybenzene
-
-
-
?
S-adenosyl-L-methionine + 2,4-dichlorophenol
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 2,4-dichloro-1-methoxybenzene
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxyacetophenone
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4,5-trimethoxyacetophenone
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzaldehyde
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzaldehyde
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzoic acid
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzoic acid
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3-methoxyacetophenone
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4-dimethoxyacetophenone
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzaldehyde
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4-dimethoxybenzaldehyde
-
-
-
?
S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzoic acid
-
741668
Phanerochaete chrysosporium
S-adenosyl-L-homocysteine + 3,4-dimethoxybenzoic acid
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 54000, SDS-PAGE
Phanerochaete chrysosporium
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
rapid inactivation above
Phanerochaete chrysosporium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
-
Phanerochaete chrysosporium
Other publictions for EC 2.1.1.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741668
Coulter
Purification and properties o ...
Phanerochaete chrysosporium
Appl. Environ. Microbiol.
59
706-711
1993
-
-
-
-
-
-
7
2
-
-
1
-
-
2
-
-
1
-
-
-
1
2
9
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
7
1
2
-
-
1
-
-
-
-
1
-
-
1
2
9
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
485241
Pazmino
Inhibition of human erythrocyt ...
Homo sapiens
Clin. Pharmacol. Ther.
26
464-472
1979
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485242
Pazmino
Human erythrocyte phenol O-met ...
Homo sapiens
Clin. Chim. Acta
89
317-329
1978
-
-
-
-
-
-
-
3
1
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485240
Axelrod
Phenol-O-methyltransferase ...
Cavia porcellus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Biochim. Biophys. Acta
159
472-478
1968
-
-
-
-
-
-
4
-
1
-
-
-
-
4
-
-
-
-
-
8
-
-
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
8
-
-
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-