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Literature summary for 2.1.1.249 extracted from

  • Bose, A.; Pritchett, M.A.; Rother, M.; Metcalf, W.W.
    Differential regulation of the three methanol methyltransferase isozymes in Methanosarcina acetivorans C2A (2006), J. Bacteriol., 188, 7274-7283.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
additional information analysis of the metal content of MtbB1 by plasma emission spectroscopy revealed no detectable cobalt with a lower limit of detection for cobalt of 0.04 mol of cobalt/mol of polypeptide Methanosarcina barkeri
Zn2+ 0.43 mol of zinc/mol of polypeptide Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
x * 51000, SDS-PAGE Methanosarcina barkeri
230000
-
gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
?
additional information Methanosarcina barkeri MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine. A MtbB1to corrinoid protein MtbC ratio of 1 is optimal for coenzyme M methylation with dimethylamine. MtbB1 methylated either corrinoid bound to MtbC or free cob(I)alamin with dimethylamine, indicating MtbB1 carries an active site for dimethylamine demethylation and corrinoid methylation ?
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
gene mtbB1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 35.2fold MtbB1 by two steps of anion exchange chromatography, and adsorption and hydrophobic interaction chromatography Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.89
-
purified enzyme, pH 6.5, 23°C Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
?
additional information MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine. A MtbB1to corrinoid protein MtbC ratio of 1 is optimal for coenzyme M methylation with dimethylamine. MtbB1 methylated either corrinoid bound to MtbC or free cob(I)alamin with dimethylamine, indicating MtbB1 carries an active site for dimethylamine demethylation and corrinoid methylation Methanosarcina barkeri ?
-
?
additional information dimethylamine-dependent methylation of free cobalamin by MtbB1, kinetics, overview Methanosarcina barkeri ?
-
?

Subunits

Subunits Comment Organism
oligomer x * 51000, SDS-PAGE Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
dimethylamine: corrinoid methyltransferase
-
Methanosarcina barkeri
DMA:corrinoid methyltransferase
-
Methanosarcina barkeri
MtbB1
-
Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Methanosarcina barkeri

General Information

General Information Comment Organism
metabolism highly purified dimethylamine: corrinoid methyltransferase MtbB1, corrinoid protein MtbC, and methylcorrinoid:coenzyme M methyltransferase MtbA, EC 2.1.1.247, are sufficient to catalyze in vitro CoM methylation by DMA Methanosarcina barkeri
additional information MtbB1 is a 230-kDa protein composed of 51-kDa subunits that do not possess a corrinoid prosthetic group Methanosarcina barkeri