Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RamA | a 60-kDa monomeric iron sulfur protein, is a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea, it is required for in vitro ATP-dependent reductive activation of methylamine:CoM methyl transfer mediating the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the monomethylamine corrinoid protein, MtmC, overview. RamA affects both lag times and rates during assay of MMA:CoM methyl transfer | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylamine + a [Co(I) methylamine-specific corrinoid protein] | Methanosarcina barkeri | catalytic cycle of MMA:CoM methyl transfer, overview | a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native MtmB from monomethylamine-grown cells, by two different steps of anionexchange chromatography, followed by gel filtration and another step of anion exchange chromatography to near homogeneity, co-purification with the monomethylamine-specific corrinoid protein MtmC | Methanosarcina barkeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:monomethylamine-grown cell | - |
Methanosarcina barkeri | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylamine + a [Co(I) methylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia | - |
? | |
methylamine + a [Co(I) methylamine-specific corrinoid protein] | catalytic cycle of MMA:CoM methyl transfer, overview | Methanosarcina barkeri | a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia | - |
? |
Synonyms | Comment | Organism |
---|---|---|
corrinoid-dependent methylamine methyltransferase | - |
Methanosarcina barkeri |
MMA:CoM methyltransferase | - |
Methanosarcina barkeri |
MtmB | - |
Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
metabolism | archaeal methane formation from methylamines is initiated by distinct methyltransferases with specificity for monomethylamine, dimethylamine, or trimethylamine. Each methylamine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by a second methyltransferase to form methyl-coenzyme M, the direct methane precursor | Methanosarcina barkeri |