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Literature summary for 2.1.1.248 extracted from

  • Ferguson, T.; Soares, J.; Lienard, T.; Gottschalk, G.; Krzycki, J.
    RamA, a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea (2009), J. Biol. Chem., 284, 2285-2295.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
RamA a 60-kDa monomeric iron sulfur protein, is a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea, it is required for in vitro ATP-dependent reductive activation of methylamine:CoM methyl transfer mediating the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the monomethylamine corrinoid protein, MtmC, overview. RamA affects both lag times and rates during assay of MMA:CoM methyl transfer Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri catalytic cycle of MMA:CoM methyl transfer, overview a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
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?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
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-
-

Purification (Commentary)

Purification (Comment) Organism
native MtmB from monomethylamine-grown cells, by two different steps of anionexchange chromatography, followed by gel filtration and another step of anion exchange chromatography to near homogeneity, co-purification with the monomethylamine-specific corrinoid protein MtmC Methanosarcina barkeri

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:monomethylamine-grown cell
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Methanosarcina barkeri
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylamine + a [Co(I) methylamine-specific corrinoid protein]
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Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
?
methylamine + a [Co(I) methylamine-specific corrinoid protein] catalytic cycle of MMA:CoM methyl transfer, overview Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
?

Synonyms

Synonyms Comment Organism
corrinoid-dependent methylamine methyltransferase
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Methanosarcina barkeri
MMA:CoM methyltransferase
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Methanosarcina barkeri
MtmB
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Methanosarcina barkeri

General Information

General Information Comment Organism
metabolism archaeal methane formation from methylamines is initiated by distinct methyltransferases with specificity for monomethylamine, dimethylamine, or trimethylamine. Each methylamine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by a second methyltransferase to form methyl-coenzyme M, the direct methane precursor Methanosarcina barkeri