Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein] | - |
? | |
a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein] | - |
? | |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
isozymes MT2-M and MT2-A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein] | - |
? | |
a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein] | - |
? | |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methylcobamide:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
MT2-A | - |
Methanosarcina barkeri |
MT2-M | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
malfunction | immunosorptive depletion of MT2 isozymes from cell-free extracts, extracts of methanol-grown cells depleted of MT2-M lose entirely the ability to carry out conversion of methanol to 2-(methylthio)ethanesulfonate, i.e. methyl-CoM. Methanol:CoM methyl transfer activity is completely restored by addition of purified MT2-M, but no activity is recovered by addition of MT2-A. In contrast, the activity of trimethylamine-grown cell extracts to convert monomethylamine and dimethylamine to methyl-CoM is lost almost entirely by immunosorptive removal of MT2-A. Addition of purified MT2-A but not MT2-M, to the MT2-A-depleted extract fully reconstitutes methyl-CoM formation from both mono- and dimethylamine | Methanosarcina barkeri |
metabolism | MT2-A plays a specific role in metabolism of methylated amine substrates, whereas, MT2-M functions in methane formation from trimethylamine and methanol, while neither of the two MT2 isozymes is involved in methane formation from acetate | Methanosarcina barkeri |