Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methyl viologen | strictly dependent on | Methanosarcina barkeri | |
additional information | stimulation of the TMA:CoM methyl transfer reaction in cell extracts | Methanosarcina barkeri | |
Ti(III) citrate | strictly dependent on | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
1 * 52000, SDS-PAGE | Methanosarcina barkeri |
52900 | - |
gel filtration and native PAGE | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanosarcina barkeri | corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | O30640 | amine isozyme MT2-A, gene mtbA | - |
Purification (Comment) | Organism |
---|---|
native enzyme by two different steps of anion exchange chromatography, gel filtration, and another step of anion exchange chromatography, co-purification with the 26-kDa the trimethylamine-specific corrinoid protein | Methanosarcina barkeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:trimethylamine-grown cell | - |
Methanosarcina barkeri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
isozyme MT2-A, pH 7.0, 37°C | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M | trimethylamine methyl transfer required, neither dimethylamine nor monomethylamine serve as substrate | Methanosarcina barkeri | methyl-CoM + a [Co(I) methylamine-specific corrinoid protein] | - |
? | |
additional information | corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction | Methanosarcina barkeri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
methylcorrinoid:CoM methyltransferase | - |
Methanosarcina barkeri |
methyltransferase II | - |
Methanosarcina barkeri |
MT2 | - |
Methanosarcina barkeri |
TMA:CoM methyltransferase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
metabolism | involvement of MT2-A in monomethylamine metabolism | Methanosarcina barkeri |