Cloned (Comment) | Organism |
---|---|
gene mrsA, DNA and amino acid sequence determination and analysis, sequences of genes mrsA and mrsB reveal an overlapping region in the start/stop sequences, sequence comparisons, mrsA-ATGA-mrsB, overexpression of the His-tagged MrsA in Escherichia coli strain BL21(DE3) | Pseudomonas syringae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Tn5 transposon mutagenesis | Pseudomonas syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate | Pseudomonas syringae | - |
S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | D5FKJ3 | pv. syringae 22d/93 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MrsA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate | - |
Pseudomonas syringae | S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate | - |
? | |
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate | - |
Pseudomonas syringae | S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate | NMR and mass spectrometric product analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
mrsA | - |
Pseudomonas syringae |
S-adenosylmethionine-dependent methyltransferase | - |
Pseudomonas syringae |
SAM-dependent methyltransferase | - |
Pseudomonas syringae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Pseudomonas syringae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Pseudomonas syringae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Pseudomonas syringae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine | Pseudomonas syringae |
physiological function | the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine, which is highly active against the closely related soybean pathogen Pseudomonas syringae pv. glycinea. Since these pathogens compete for the same habitat, Pss22d is a promising candidate for biocontrol of P. syringae pv. glycinea | Pseudomonas syringae |