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Literature summary for 2.1.1.240 extracted from

  • Kallscheuer, N.; Vogt, M.; Bott, M.; Marienhagen, J.
    Functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin (2017), J. Biotechnol., 258, 190-196 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis introduction of enzyme into a resveratrol-producing Corynebacterium glutamicum strain allows synthesis of 42 mg/l (0.16 mM) of the di-O-methylated pterostilbene from p-coumaric acid. A fusion of O-methyltransferase with the maltose-binding protein of Escherichia coli lacking its signal peptide increases the solubility of the O-methyltransferase. Expression of heterologous dioxygenase genes in (2S)-flavanone-producing Corynebacterium glutamicum strains enables the production of flavanonols and flavonols starting from p-coumaric acid and caffeic acid. For the flavonols kaempferol and quercetin, maximum product titers of 23 mg/l (0.08 mM) and 10 mg/l (0.03 mM) can be achieved Vitis vinifera
synthesis the enzyme is used for production of large amounts of easily recoverable extracellular resveratrol in a bacterial system, method, overview. Constitutive expression of either Vitis vinifera resveratrol O-methyltransferase (VvROMT) or human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) lead to pterostilbene or piceatannol, respectively, after the engineered cell cultures are treated with elicitors. i.e. methylated cyclodextrins and methyl jasmonate. Functionality of both gene products is first assessed in planta by Nicotiana benthamiana agroinfiltration assays, in which tobacco cells transiently express stilbene synthase and VvROMT or HsCYP1B1 Vitis vinifera

Cloned(Commentary)

Cloned (Comment) Organism
gene ROMT, recombinant expression in Corynebacterium glutamicum and in Vitis vinifera cell culture, coexpression with flavanone 3-hydroxylase, and flavonol synthase, transient recombinant expression of HA-tagged enzyme in Nicotiana benthamiana leaves via Agrobacterium-mediated transient transformation, coexpression with human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) Vitis vinifera

Protein Variants

Protein Variants Comment Organism
additional information functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum and in Vitis vinifera cell culture for production of pterostilbene, kaempferol, and quercetin Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + trans-resveratrol Vitis vinifera
-
 2 S-adenosyl-L-homocysteine + pterostilbene
-
 ?

Organism

Organism UniProt Comment Textmining
Vitis vinifera B6VJS4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
berry
-
 Vitis vinifera
-
cell culture
-
 Vitis vinifera
-
fruit
-
 Vitis vinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + trans-resveratrol
-
 Vitis vinifera 2 S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2 S-adenosyl-L-methionine + trans-resveratrol
-
 Vitis vinifera 2 H+ + pterostilbene + 2 S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
MT
-
 Vitis vinifera
ROMT
-
 Vitis vinifera
VvRO
-
 Vitis vinifera

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Vitis vinifera

Expression

Organism Comment Expression
Vitis vinifera methylated cyclodextrins and methyl jasmonate induce the enzyme, accumulation of stilbenoids in grapevine cell cultures upon elicitation up