Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for conversion of 4-coumaric acid to pterostilbene via co-expression of 4CL::STS and ROMT in recombinant Escherichia coli and Saccharomyces cerevisiae, overview | Vitis vinifera |
Cloned (Comment) | Organism |
---|---|
gene ROMT, recombinant expression in Escherichia coli strain BW27784 and in Saccharomyces cerevisiae, coexpression of two additional genes from the resveratrol biosynthetic pathway, 4-coumarate CoA-ligase and stilbene synthase, leads to production of a large amount of pterostilbene with a trace amount of pinostilbene, subcloning in Escherichia coli strain DH5alpha, recombinant expression of grape SUMO-fusion wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Vitis vinifera |
Protein Variants | Comment | Organism |
---|---|---|
D174A | site-directed mutagenesis, almost inactive mutant | Vitis vinifera |
F167A | site-directed mutagenesis, inactive mutant | Vitis vinifera |
H261A | site-directed mutagenesis, inactive mutant | Vitis vinifera |
W258A | site-directed mutagenesis, inactive mutant | Vitis vinifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, recombinant SUMO-ROMT enzyme, SDS-PAGE | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | Vitis vinifera | i.e. pinostilbene | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | Vitis vinifera | - |
S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | B6VJS4 | gene ROMT | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | i.e. pinostilbene | Vitis vinifera | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | - |
Vitis vinifera | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant SUMO-ROMT enzyme, SDS-PAGE | Vitis vinifera |
Synonyms | Comment | Organism |
---|---|---|
resveratrol O-methyltransferase | - |
Vitis vinifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Vitis vinifera |
General Information | Comment | Organism |
---|---|---|
additional information | three-dimensional enzyme homology modeling and docking study for identification of four key catalytic residues. Residues F167 and W258 form a sandwich to bind resveratrol, residue D174 is in close proximity to the substrate, and residue H261 might serve as a general base in the deprotonation of hydroxyl groups | Vitis vinifera |