Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique results in the accumulation of pterostilbene in tobacco tissues | Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
resveratrol | pH 7.5, temperature not specified in the publication | Vitis vinifera | |
0.014 | - |
3-methoxy-4',5-dihydroxy-trans-stilbene | pH 7.5, temperature not specified in the publication | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + resveratrol | Vitis vinifera | the enzyme catalyses the biosynthesis of pterostilbene from resveratrol | 2 S-adenosyl-L-homocysteine + pterostilbene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vitis vinifera |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene | overall reaction | Vitis vinifera | |
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene | (1b) | Vitis vinifera | |
S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | (1a) | Vitis vinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Vitis vinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + resveratrol | - |
Vitis vinifera | 2 S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
2 S-adenosyl-L-methionine + resveratrol | the enzyme catalyses the biosynthesis of pterostilbene from resveratrol | Vitis vinifera | 2 S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | resveratrol monomethyl ether i.e. 3-methoxy-4',5-dihydroxy-trans-stilbene | Vitis vinifera | S-adenosyl-L-homocysteine + pterostilbene | pterostilbene i.e. 3,5-dimethoxy-4'-hydroxy-trans-stilbene | ? | |
S-adenosyl-L-methionine + resveratrol | - |
Vitis vinifera | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | resveratrol monomethyl ether i.e. 3-methoxy-4',5-dihydroxy-trans-stilbene | ? |
Synonyms | Comment | Organism |
---|---|---|
resveratrol O-methyltransferase | - |
Vitis vinifera |
ROMT | - |
Vitis vinifera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
resveratrol | pH 7.5, temperature not specified in the publication | Vitis vinifera | |
0.086 | - |
3-methoxy-4',5-dihydroxy-trans-stilbene | pH 7.5, temperature not specified in the publication | Vitis vinifera |
Organism | Comment | Expression |
---|---|---|
Vitis vinifera | ROMT gene expression in grapevine leaves is induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl3 treatment | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.16 | - |
resveratrol | pH 7.5, temperature not specified in the publication | Vitis vinifera | |
6.14 | - |
3-methoxy-4',5-dihydroxy-trans-stilbene | pH 7.5, temperature not specified in the publication | Vitis vinifera |