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Literature summary for 2.1.1.240 extracted from

  • Schmidlin, L.; Poutaraud, A.; Claudel, P.; Mestre, P.; Prado, E.; Santos-Rosa, M.; Wiedemann-Merdinoglu, S.; Karst, F.; Merdinoglu, D.; Hugueney, P.
    A stress-inducible resveratrol O-methyltransferase involved in the biosynthesis of pterostilbene in grapevine (2008), Plant Physiol., 148, 1630-1639.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique results in the accumulation of pterostilbene in tobacco tissues Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
resveratrol pH 7.5, temperature not specified in the publication Vitis vinifera
0.014
-
3-methoxy-4',5-dihydroxy-trans-stilbene pH 7.5, temperature not specified in the publication Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + resveratrol Vitis vinifera the enzyme catalyses the biosynthesis of pterostilbene from resveratrol 2 S-adenosyl-L-homocysteine + pterostilbene
-
?

Organism

Organism UniProt Comment Textmining
Vitis vinifera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vitis vinifera

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene overall reaction Vitis vinifera
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene (1b) Vitis vinifera
S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene (1a) Vitis vinifera

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Vitis vinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + resveratrol
-
Vitis vinifera 2 S-adenosyl-L-homocysteine + pterostilbene
-
?
2 S-adenosyl-L-methionine + resveratrol the enzyme catalyses the biosynthesis of pterostilbene from resveratrol Vitis vinifera 2 S-adenosyl-L-homocysteine + pterostilbene
-
?
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene resveratrol monomethyl ether i.e. 3-methoxy-4',5-dihydroxy-trans-stilbene Vitis vinifera S-adenosyl-L-homocysteine + pterostilbene pterostilbene i.e. 3,5-dimethoxy-4'-hydroxy-trans-stilbene ?
S-adenosyl-L-methionine + resveratrol
-
Vitis vinifera S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene resveratrol monomethyl ether i.e. 3-methoxy-4',5-dihydroxy-trans-stilbene ?

Synonyms

Synonyms Comment Organism
resveratrol O-methyltransferase
-
Vitis vinifera
ROMT
-
Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.062
-
resveratrol pH 7.5, temperature not specified in the publication Vitis vinifera
0.086
-
3-methoxy-4',5-dihydroxy-trans-stilbene pH 7.5, temperature not specified in the publication Vitis vinifera

Expression

Organism Comment Expression
Vitis vinifera ROMT gene expression in grapevine leaves is induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl3 treatment up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.16
-
resveratrol pH 7.5, temperature not specified in the publication Vitis vinifera
6.14
-
3-methoxy-4',5-dihydroxy-trans-stilbene pH 7.5, temperature not specified in the publication Vitis vinifera