Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a knockout lcmt1 mutant sbi1, phenotype, overview. PP2A-C methylation status in wild-type and lcmt1 mutant seedlings under control conditions | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine | Arabidopsis thaliana Col-0 | - |
S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VY08 | - |
- |
Arabidopsis thaliana Col-0 | Q8VY08 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme expression analysis in wild-type and mutant plant organs, overview | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
rosette leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine | - |
Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LCMT-1 | - |
Arabidopsis thaliana |
leucine carboxyl methyltransferase 1 homolog | UniProt | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the lcmt1 knockout mutant possesses essentially only unmethylated PP2A-C, has less dense rosettes, and earlier flowering compared to wild-type plants. Approximately 200 overlapping genes are 2fold upregulated, and 200 overlapping genes are 2fold downregulated in both lcmt1 and pme1 knockout mutants compared to wild-type. Differences between the 2 mutants, 97 genes are 2fold upregulated in pme1 compared with lcmt1, indicating that PME1 acts as a negative regulator for these genes. Arabidopsis thaliana plants with knocked out LCMT1 (sbi1 mutant) are void of methylated PP2A-C and have unmethylated catalytic subunit PP2A-C both in the microsomal and cytosolic fractions | Arabidopsis thaliana |
metabolism | protein phosphatase 2A catalytic subunit (PP2A-C) has a terminal leucine subjected to methylation, a regulatory mechanism conserved from yeast to mammals and plants. Two enzymes, leucine carboxyl methyltransferase (LCMT-1) and protein phosphatase methylesterase (PME-1, EC 3.1.1.89), methylate and demethylate PP2A-C, respectively, physiological importance of these posttranslational modifications. Regulation, overview. In addition to catalyzing demethylation of PP2A-C, PME1 has a function in stabilizing the PP2A complex, keeping part of the PP2AC pool in an inactive state | Arabidopsis thaliana |
physiological function | protein phosphatase 2A catalytic subunit (PP2A-C) has a terminal leucine subjected to methylation, a regulatory mechanism conserved from yeast to mammals and plants. Two enzymes, leucine carboxyl methyltransferase (LCMT-1) and protein phosphatase methylesterase (PME-1, EC 3.1.1.89), methylate and demethylate PP2A-C, respectively, physiological importance of these posttranslational modifications. Active LCMT1 methylates all PP2A-C in microsomes | Arabidopsis thaliana |