Cloned (Comment) | Organism |
---|---|
gene CrOMT6, DNA and amino acid sequence determination and analysis, qualitative RT-PCR expression analysis, sequence comparison and phylogenetic tree, expression of His-tagged OMT6 in Escherichia coli | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3'-O-methyleriodictyol | Catharanthus roseus | - |
S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | Q6VCW3 | gene CrOMT6 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged OMT6 in Escherichia coli by nickel affinity chromatgraphy | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Catharanthus roseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the only substrates found for CrOMT6 are 3'-O-methyl-eriodictyol (homoeriodictyol) and the corresponding flavones and flavonols, substrate specificty, overview. No or poor activity with naringenin, pentahydroxyflavanone, hesperetin, myricetin, 7,3'-O-dimethylquercetin, 7-O-methylquercetin, syringetin, apigenin, luteolin, tricetin, velutin, dihydrokaempferol, dihydroquercetin, dihydromyricetin, and 3'-O-methyl-dihydroquercetin, and also no activity with ferulic acid, coniferyl alcohol, vanillic acid vanillin, eugenol, isoeugenol, and guaicol. Not only the B-ring configuration, but also the size and shape of the A-ring are critical parts of the substrate specificity | Catharanthus roseus | ? | - |
? | |
S-adenosyl-L-methionine + 3'-O-methyleriodictyol | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol | - |
? | |
S-adenosyl-L-methionine + 3'-O-methyleriodictyol | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol | product identification by mass spectrometry | ? | |
S-adenosyl-L-methionine + chrysoeriol | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 4'-O-methylchrysoeriol | - |
? | |
S-adenosyl-L-methionine + eriodictyol | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 4'-O-methyleriodictyol | - |
? | |
S-adenosyl-L-methionine + isorhamnetin | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 4'-O-methylisorhamnetin | - |
? | |
S-adenosyl-L-methionine + kaempferol | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 4'-O-methylkaempferol | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Catharanthus roseus | S-adenosyl-L-homocysteine + 4'-O-methylquercetin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4'-O-methyltransferase | - |
Catharanthus roseus |
4'OMT | - |
Catharanthus roseus |
B-ring 4'OMT | - |
Catharanthus roseus |
flavonoid 4'-O-methyltransferase | UniProt | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Catharanthus roseus |