Literature summary for 2.1.1.230 extracted from
Thompson, J.; Cundliffe, E.
Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton (1981), J. Gen. Microbiol., 124, 291-297.
No PubMed abstract available
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
S-adenosyl-L-homocysteine |
- |
Streptomyces azureus |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.15 |
- |
S-adenosyl-L-methionine |
pH 7.5, 35°C |
Streptomyces azureus |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
K+ |
ionic optimum: 50-100 mM |
Streptomyces azureus |
|
Mg2+ |
ionic optimum: 5-20 mM |
Streptomyces azureus |
|
NH4+ |
ionic optimum: 50-100 mM |
Streptomyces azureus |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
35000 |
38000 |
gel filtration |
Streptomyces azureus |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
Streptomyces azureus |
the enzyme is involved in resistance to thiostrepton |
S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Streptomyces azureus |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
- |
Streptomyces azureus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
- |
Streptomyces azureus |
S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA |
- |
? |
|
S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
the enzyme is involved in resistance to thiostrepton |
Streptomyces azureus |
S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
thiostrepton-resistance methylase |
- |
Streptomyces azureus |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
35 |
- |
assay at |
Streptomyces azureus |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
30 |
45 |
quite active from 30°C-45°C |
Streptomyces azureus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
7.6 |
- |
Streptomyces azureus |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
7 |
7.8 |
pH 7.0: about 60% of maximal activity, pH 7.8: about 60% of maximal activity |
Streptomyces azureus |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
6 |
- |
unstable below |
Streptomyces azureus |
Ki Value [mM]
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
0.0005 |
- |
S-adenosyl-L-homocysteine |
pH 7.5, 35°C |
Streptomyces azureus |
|
General Information
General Information |
Comment |
Organism |
physiological function |
the enzyme is involved in resistance to thiostrepton |
Streptomyces azureus |