Cloned (Comment) | Organism |
---|---|
gene At3g56120, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21, recombinant expression in and full functional complementation of Saccgaromyces cerevisiae DELTAtrm5 mutant with the AtTRM5a gene. Yeast GBY15 mutant is defective for m1G37 nucleoside modification, and the mutant strain grows slow compared to the congenic strain GBY16 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of T-DNA insertion attrm5a deletion mutants, phenotypes, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC) | Arabidopsis thaliana | enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93YU6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
rosette leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC) | enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro | Arabidopsis thaliana | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At3g56120 | - |
Arabidopsis thaliana |
AtTrm5a | - |
Arabidopsis thaliana |
tRNA (guanine(37)-N1)-methyltransferase 1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | the AdoMet binding pocket is located between E206, R209, N380, P382, Y198 and R165. Due to the flexibility of D1 domain, a structure model is projected using only D2 and D3 domains, using structure PDB 2YX1 of chain A of Trm5 from Methanococcus jannashii as template | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | Arabidopsis attrm5a mutants are dwarfed and have short filaments, which leads to reduced seed setting. Proteomics data indicate differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate are reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaires m1G and m1I methylation and leads to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants | Arabidopsis thaliana |
physiological function | modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37, a function mediated by Trm5 homologues. Enzyme AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana. The importance of m1G37 is highlighted by its direct function in the decoding process | Arabidopsis thaliana |