Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a fusion protein with maltose-binding protein | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
mitochondrial matrix | - |
Saccharomyces cerevisiae | 5759 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Saccharomyces cerevisiae | Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38793 | - |
- |
Purification (Comment) | Organism |
---|---|
Trm5p is purified as a fusion protein with maltose-binding protein | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | methyltransferase activity with tRNA isolated from a DELTAtrm5 mutant strain, as well as with a synthetic mitochondrial initiator tRNA (tRNAMetf). N1-Methylguanine is determined by high pressure liquid chromatography analysis | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | the site of methylation is guanosine 37 in both mitochondrial tRNAMetf and tRNAPhe, determined by primer extension | ? |
Synonyms | Comment | Organism |
---|---|---|
Trm5p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | efficiency or accuracy of mitochondrial protein synthesis is decreased in cells lacking m1G37 methylation of mitochondrial tRNAs | Saccharomyces cerevisiae |
physiological function | the m1G37 tRNA modification plays an important role in reading frame maintenance in mitochondrial protein synthesis | Saccharomyces cerevisiae |