Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-values for truncated tRNAPhe variants | Aquifex aeolicus | |
0.0004 | - |
guanine37 in Aquifex aeolicus tRNAGln(UUG) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0005 | - |
guanine37 in Haloferax volcanii tRNALeu(CAA) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0006 | - |
guanine37 in Aquifex aeolicus tRNAHis(GUG) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0006 | - |
guanine37 in Aquifex aeolicus tRNAArg(ACG) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0007 | - |
guanine37 in Aquifex aeolicus tRNAPro(GGG) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0008 | - |
guanine37 in Aquifex aeolicus tRNAArg(CCG) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0008 | - |
guanine37 in yeast tRNAPhe(GAA) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0009 | - |
guanine37 in Aquifex aeolicus tRNALeu(CAG) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0019 | - |
guanine37 in Haloferax volcanii tRNATyr(GUA) | pH 7.6, 60°C | Aquifex aeolicus | |
0.002 | - |
guanine37 in Haloferax volcanii tRNACys(GCA) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0023 | - |
guanine37 in Haloferax volcanii tRNATrp(CCA) | pH 7.6, 60°C | Aquifex aeolicus | |
0.0033 | - |
guanine37 in in Escherichia coli tRNALeu(CAG) | pH 7.6, 60°C | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Aquifex aeolicus | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with guanine37 in yeast tRNAAsp(GUC) possessing a C36G37 sequence, guanine37 in Haloferax volcanii tRNAGlu(UUC) possessing a C36G37 sequence, guanine37 in yeast tRNAPhe A36U mutant(GAU) possessing a U36G37 sequence, guanine37 in Escherichia coli tRNASer(UGA) possessing a A36G37 sequence | Aquifex aeolicus | ? | - |
? | |
S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAArg(ACG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAArg(ACG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAArg(CCG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAArg(CCG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAGln(UUG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAGln(UUG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAHis(GUG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAHis(GUG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNALeu(CAG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNALeu(CAG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAPro(GGG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAPro(GGG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNACys(GCA) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNACys(GCA) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNALeu(CAA) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNALeu(CAA) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNATrp(CCA) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNATrp(CCA) | - |
? | |
S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNATyr(GUA) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNATyr(GUA) | - |
? | |
S-adenosyl-L-methionine + guanine37 in in Escherichia coli tRNALeu(CAG) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in in Escherichia coli tRNALeu(CAG) | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | the enzyme methylates tRNA transcripts possessing an A36G37 sequence as well as tRNA transcripts possessing a G36G37 sequence. tRNA transcripts possessing pyrimidine36G37 are not methylated at all. The modified nucleoside and the position in yeast tRNA(Phe) transcript are confirmed by LC/MS. Nine truncated tRNA molecules are tested to clarify the additional recognition site. The TrmD protein efficiently methylates the micro helix corresponding to the anti-codon arm. Because the disruption of the anti-codon stem causes the complete loss of the methyl group acceptance activity, the anti-codon stem is essential for the recognition. The existence of the D-arm structure inhibits the activity | Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in yeast tRNAPhe(GAA) | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAPhe(GAA) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
transfer RNA (m1G37) methyltransferase | - |
Aquifex aeolicus |
TrmD | - |
Aquifex aeolicus |
tRNA (m1G37) methyltransferase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Aquifex aeolicus |