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Literature summary for 2.1.1.225 extracted from

  • Wilkinson, M.L.; Crary, S.M.; Jackman. J.E.; Grayhack, E.J.; Phizicky, E.M.
    The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4 (2007), RNA, 13, 404-413.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
adenosine4 in tRNAHis pH 8.0, 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenosine4 in tRNAHis Saccharomyces cerevisiae the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) Saccharomyces cerevisiae the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro Saccharomyces cerevisiae the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12383
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + adenosine4 in tRNAHis = S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis (3) Saccharomyces cerevisiae
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC) (2) Saccharomyces cerevisiae
S-adenosyl-L-methionine + cytidine4 in tRNAPro = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro (1) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenosine4 in tRNAHis
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
?
S-adenosyl-L-methionine + adenosine4 in tRNAHis the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
?

Synonyms

Synonyms Comment Organism
TRM13
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
adenosine4 in tRNAHis pH 8.0, 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction trm13-DELTA strains do not have an obvious growth defect Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
400
-
adenosine4 in tRNAHis pH 8.0, 30°C Saccharomyces cerevisiae