Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
adenosine4 in tRNAHis | pH 8.0, 30°C | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenosine4 in tRNAHis | Saccharomyces cerevisiae | the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA | S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis | - |
? | |
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) | Saccharomyces cerevisiae | the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA | S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC) | - |
? | |
S-adenosyl-L-methionine + cytidine4 in tRNAPro | Saccharomyces cerevisiae | the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA | S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12383 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + adenosine4 in tRNAHis = S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis | (3) | Saccharomyces cerevisiae | |
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC) | (2) | Saccharomyces cerevisiae | |
S-adenosyl-L-methionine + cytidine4 in tRNAPro = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro | (1) | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenosine4 in tRNAHis | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis | - |
? | |
S-adenosyl-L-methionine + adenosine4 in tRNAHis | the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis | - |
? | |
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC) | - |
? | |
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) | the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC) | - |
? | |
S-adenosyl-L-methionine + cytidine4 in tRNAPro | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro | - |
? | |
S-adenosyl-L-methionine + cytidine4 in tRNAPro | the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRM13 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
adenosine4 in tRNAHis | pH 8.0, 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | trm13-DELTA strains do not have an obvious growth defect | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
adenosine4 in tRNAHis | pH 8.0, 30°C | Saccharomyces cerevisiae |