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Literature summary for 2.1.1.224 extracted from

  • Candela, T.; Marvaud, J.; Nguyen, T.; Lambert, T.
    A cfr-like gene cfr(C) conferring linezolid resistance is common in Clostridium difficile (2017), Int. J. Antimicrob. Agents, 50, 496-500 .
    View publication on PubMed
Search for more literature for 2.1.1.224

Cloned(Commentary)

Cloned (Comment) Organism
gene cfr(C), DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, cfr(C) is constitutively transcribed, gene cfr(C) is part of a putative 24 kb-transposon, which generates a detectable circular intermediate Enterocloster bolteae
gene cfr(C), DNA and amino acid sequence determination and analysis, the cfr-like is cloned in Clostridium difficile strain 630DELTAerm in which it confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A (PhLOPSA) Clostridioides difficile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin Clostridioides difficile
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin Enterocloster bolteae
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin Clostridioides difficile T10
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin Enterocloster bolteae 90B3
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridioides difficile V5ZEQ2 the strain is resistant to erythromycin, chloramphenicol, clindamycin, florfenicol, linezolid, streptogramin A, and tiamulin
-
Clostridioides difficile T10 V5ZEQ2 the strain is resistant to erythromycin, chloramphenicol, clindamycin, florfenicol, linezolid, streptogramin A, and tiamulin
-
Enterocloster bolteae
-
-
-
Enterocloster bolteae 90B3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
-
 Clostridioides difficile S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
-
 Enterocloster bolteae S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
-
 Clostridioides difficile T10 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
-
 Enterocloster bolteae 90B3 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
 ?

Synonyms

Synonyms Comment Organism
antibiotic resistance protein
-
 Clostridioides difficile
antibiotic resistance protein
-
 Enterocloster bolteae
Cfr
-
 Clostridioides difficile
Cfr
-
 Enterocloster bolteae
cfr(C)
-
 Clostridioides difficile
cfr-like
-
 Clostridioides difficile
radical S-adenosylmethionine methylase
-
 Clostridioides difficile
radical S-adenosylmethionine methylase
-
 Enterocloster bolteae
RS methylase
-
 Clostridioides difficile
RS methylase
-
 Enterocloster bolteae

Cofactor

Cofactor Comment Organism Structure
reduced [2Fe-2S] ferredoxin
-
 Clostridioides difficile
reduced [2Fe-2S] ferredoxin
-
 Enterocloster bolteae
S-adenosyl-L-methionine
-
 Clostridioides difficile
S-adenosyl-L-methionine
-
 Enterocloster bolteae

Expression

Organism Comment Expression
Enterocloster bolteae gene cfr(C) is similarly expressed in absence or presence of either chloramphenicol, clindamycin, or linezolid additional information

General Information

General Information Comment Organism
evolution cfr(C) is part of a putative 24 kb-transposon, which generated a detectable circular intermediate. An element differing by a single nucleotide is found in Clostridium difficile DA00203 from GenBank data, consistent with a recent horizontal transfer Enterocloster bolteae
additional information gene cfr(C) is mainly confined in Clostridium difficile within polymorphic environments suggesting that this microorganism is a reservoir for PhLOPSA resistance. In silico analysis shows cfr(C) in 19 out of 274 Clostridium difficile genomes. This gene is also detected by PCR analysis in 9 out of 80 Clostridium difficile from a laboratory strain collection according to resistance to linezolid and florfenicol Clostridioides difficile
physiological function Clostridium bolteae strain 90B3 is multiresistant to various antibiotics including ampicillin, as well as to linezolid, florfenicol, streptogramin A, and tiamulin due to methylation of 23S rRNA at A2503 Enterocloster bolteae
physiological function gene cfr(C) confers linezolid resistance is common in Clostridium difficile. Clostridium difficile strain T10 is resistant to erythromycin, chloramphenicol, clindamycin, florfenicol, linezolid, streptogramin A, and tiamulin due to methylation of 23S rRNA at A2503 Clostridioides difficile