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Literature summary for 2.1.1.220 extracted from

  • Droogmans, L.; Roovers, M.; Bujnicki, J.M.; Tricot, C.; Hartsch, T.; Stalon, V.; Grosjean, H.
    Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures (2003), Nucleic Acids Res., 31, 2148-2156.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28582
-
 4 * 28582, calculation from sequence, composed of two types of subunits (Gcd14p and Gcd10p) Thermus thermophilus
150000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q8GBB2
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q8GBB2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in tRNA catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein Thermus thermophilus S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?
S-adenosyl-L-methionine + adenine58 in tRNA catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 28582, calculation from sequence, composed of two types of subunits (Gcd14p and Gcd10p) Thermus thermophilus

Synonyms

Synonyms Comment Organism
TrmI
-
 Thermus thermophilus
tRNA (m1A58) methyltransferase
-
 Thermus thermophilus
tRNA (m1A58) MTase
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermus thermophilus

General Information

General Information Comment Organism
malfunction inactivation of the Thermus thermophilus trmI gene results in a thermosensitive phenotype (growth defect at 80°C) Thermus thermophilus
physiological function role of the N1-methylation of tRNA adenosine-58 in adaptation of life to extreme temperatures Thermus thermophilus