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Literature summary for 2.1.1.217 extracted from

  • Degut, C.; Barraud, P.; Larue, V.; Tisne, C.
    Backbone resonance assignments of the m1A22 tRNA methyltransferase TrmK from Bacillus subtilis (2016), Biomol. NMR Assign., 10, 253-257 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene trmK, recombinant overexpression of N-terminally His6-tagged mutant enzyme C35S/C152S in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C35S/C152S site-directed mutagenesis, since wild-type TrmK is prone to aggregation through cysteine oxidation, a protein variant with cysteine to serine mutations (i.e. C35S and C152S) is produced, which shows abolished protein aggregation and retains full enzymatic activity Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine22 in tRNA Bacillus subtilis
-
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
?
S-adenosyl-L-methionine + adenine22 in tRNA Bacillus subtilis 168
-
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P54471
-
-
Bacillus subtilis 168 P54471
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged mutant enzyme C35S/C152S from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, followed by ultrafiltration and gel filtration Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine22 in tRNA
-
Bacillus subtilis S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
?
S-adenosyl-L-methionine + adenine22 in tRNA
-
Bacillus subtilis 168 S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
?

Subunits

Subunits Comment Organism
More backbone 1H, 15N and 13C chemical shift assignments of TrmK from Bacillus subtilis obtained by heteronuclear multidimensional NMR spectroscopy as well as its secondary structure in solution, overview Bacillus subtilis

Synonyms

Synonyms Comment Organism
TrmK
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Bacillus subtilis

General Information

General Information Comment Organism
evolution Bacillus subtilis TrmK belongs to the COG2384 (cluster of orthologous groups). The members of this family are found in Gram-negative and Gram-positive bacteria. Their sequences are well-conserved in many bacterial pathogens Bacillus subtilis
additional information NMR chemical shift mapping is used to get insight on the protein-RNA recognition mode Bacillus subtilis