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Literature summary for 2.1.1.216 extracted from

  • Kawamura, T.; Anraku, R.; Hasegawa, T.; Tomikawa, C.; Hori, H.
    Transfer RNA methyltransferases from Thermoplasma acidophilum, a thermoacidophilic archaeon (2015), Int. J. Mol. Sci., 16, 91-113 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.216

Cloned(Commentary)

Cloned (Comment) Organism
gene Ta0997, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Rosetta 2 Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum JCM 9062
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum AMRC-C165
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum ATCC 25905
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum NBRC 15155
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum JCM 9062
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum AMRC-C165
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum ATCC 25905
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum NBRC 15155
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in elongator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in elongator tRNAMet
-
 ?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in initiator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in initiator tRNAMet
-
 ?
S-adenosyl-L-methionine + guanine26 in elongator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in elongator tRNAMet
-
 ?
S-adenosyl-L-methionine + guanine26 in initiator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in initiator tRNAMet
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum JCM 9062
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum AMRC-C165
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum ATCC 25905
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum NBRC 15155
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum JCM 9062
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum AMRC-C165
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum ATCC 25905
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum NBRC 15155
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum P57706
-
-
Thermoplasma acidophilum AMRC-C165 P57706
-
-
Thermoplasma acidophilum ATCC 25905 P57706
-
-
Thermoplasma acidophilum JCM 9062 P57706
-
-
Thermoplasma acidophilum NBRC 15155 P57706
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) Rosetta 2 Thermoplasma acidophilum

Source Tissue

Source Tissue Comment Organism Textmining
additional information Thermoplasma acidophilum grows optimally at 59°C and pH 1.9 Thermoplasma acidophilum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum JCM 9062 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum JCM 9062 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum AMRC-C165 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum ATCC 25905 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum NBRC 15155 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum ?
-
-
additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum JCM 9062 ?
-
-
additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum AMRC-C165 ?
-
-
additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum ATCC 25905 ?
-
-
additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum NBRC 15155 ?
-
-
S-adenosyl-L-methionine + 2'-O-methylguanine26 in elongator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in elongator tRNAMet
-
 ?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in initiator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in initiator tRNAMet
-
 ?
S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in elongator tRNAMet
-
 ?
S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in initiator tRNAMet
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
Ta0997
-
 Thermoplasma acidophilum
transfer RNA methyltransferase
-
 Thermoplasma acidophilum
Trm1
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Thermoplasma acidophilum

General Information

General Information Comment Organism
evolution Trm1 enzymes can be divided into two types on the basis of their specificity for the target guanosine(s). One is a single-site-specific, which modifies only G26 and is found in eukaryotes and archaea. The second is a multi-site-specific Trm1, which modifies both G26 and G27 and is found in the hyperthermophilic eubacterium, Aquifex aeolicus Thermoplasma acidophilum
physiological function the Ta0997 gene product methylates the tRNALeuUAG transcript and 14C-nucleotide analysis reveals that the modified nucleotide is pm22G. Archaeal Trm1 can methylate the tRNALeuUAG transcript, which has a long variable region. In the case of class I tRNAs, archaeal Trm1 is reported to recognize the D-stem and the size of the variable region. Therefore, archaeal Trm1 might be able to recognize the large variable region in the class II tRNAs. Trm1 efficiently methylates both the mature elongator tRNAMet transcript and the precursor with an intron. Archaeal Trm1 does not recognize the anticodon loop. The G modification at position 26 is a mixture of m2G and m22G, which is formed by Trm1 Thermoplasma acidophilum