Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
K290A | mutant enzyme loses 56% of its methylation activity | Saccharomyces cerevisiae |
R246A | more than 97% loss of activity | Caenorhabditis elegans |
R246G | mutation eliminates the methylation activity | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58400 | - |
calculated from sequence | Caenorhabditis elegans |
59000 | - |
SDS-PAGE | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | Q23270 | - |
- |
Saccharomyces cerevisiae | P15565 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + guanine26 in tRNA | - |
Saccharomyces cerevisiae | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA | - |
? | |
2 S-adenosyl-L-methionine + guanine26 in tRNA | wild-type enzyme produces 88% N2-dimethylguanine26 tRNA and 12% N2-methylguanine26 in tRNA. The Caenorhabditis elegans tRNA(m22G26)dimethyltransferase efficiently recognized the Escherichia coli tRNA as a substrate in vivo, and that the enzyme is capable of both mono- and dimethylating G26 in this tRNA | Caenorhabditis elegans | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA | - |
? | |
S-adenosyl-L-methionine + guanine26 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine26 in tRNA | - |
Caenorhabditis elegans | S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA | - |
Caenorhabditis elegans | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm1p | - |
Saccharomyces cerevisiae |
tRNA(m22G26)dimethyltransferase | - |
Saccharomyces cerevisiae |
tRNA(m22G26)dimethyltransferase | - |
Caenorhabditis elegans |
ZC376.5 | - |
Caenorhabditis elegans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Caenorhabditis elegans | calculated from sequence | - |
8.7 |