Cloned (Comment) | Organism |
---|---|
gene Tk0981 | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a gene disruptant trm11 (DELTAtrm11) mutant showing the absence of m2s2G10. The lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggests that this methylation is mediated by Trm14, which is an m2G6 methyltransferase. The trm11 gene is reinserted into the chiA (Tk1765, chitinase gene) region in the genomic DNA of the DELTAtrm11 strain. Deletion of the Tk1765 gene does not cause growth defects unless chitin is used as a carbon source. Although its expression level is lower in the complemented strain than in the wild-type strain, Trm11 is expressed in the complemented strain. At 85°C, the wild-type, DELTAtrm11, and complemented strains show similar growth curves. As the temperature increases, the growth of the DELTAtrm11 strain is clearly slower than that of the wild-type or complemented strain. At 95°C, the DELTAtrm11 strain shows a considerable growth defect, whereas the complemented strain grows at approximately the same speed as the wild-type strain, indicating that the growth defect of the DELTAtrm11 strain is due to the lack of archaeal Trm11 protein | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine10 in tRNA | Thermococcus kodakarensis | - |
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | Thermococcus kodakarensis JCM 12380 | - |
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | Thermococcus kodakarensis ATCC BAA-918 | - |
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JID5 | i.e. Pyrococcus kodakaraensis strain KOD1 | - |
Thermococcus kodakarensis ATCC BAA-918 | Q5JID5 | i.e. Pyrococcus kodakaraensis strain KOD1 | - |
Thermococcus kodakarensis JCM 12380 | Q5JID5 | i.e. Pyrococcus kodakaraensis strain KOD1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | hyperthermophilic archaeon Thermococcus kodakarensis grows at 60-100°C | Thermococcus kodakarensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine10 in tRNA | - |
Thermococcus kodakarensis | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | - |
Thermococcus kodakarensis JCM 12380 | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | - |
Thermococcus kodakarensis ATCC BAA-918 | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNATrp | - |
Thermococcus kodakarensis | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNATrp | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNATrp | - |
Thermococcus kodakarensis JCM 12380 | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNATrp | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNATrp | - |
Thermococcus kodakarensis ATCC BAA-918 | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNATrp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
archaeal Trm11 | - |
Thermococcus kodakarensis |
N2, N2-dimethylguanosine tRNA methyltransferase | UniProt | Thermococcus kodakarensis |
N2,N2-dimethylguanosine tRNA methyltransferase | - |
Thermococcus kodakarensis |
Tk0981 | - |
Thermococcus kodakarensis |
Trm-G10 | - |
Thermococcus kodakarensis |
Trm-m22G10 | - |
Thermococcus kodakarensis |
Trm11 | - |
Thermococcus kodakarensis |
tRNA m2G10/m22G10 methyltransferase | - |
Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | although the eukaryotic counterpart (Trm11) requires another subunit Trm112 for enzymatic activity, the archaeal enzyme does not require a partner subunit | Thermococcus kodakarensis | |
S-adenosyl-L-methionine | - |
Thermococcus kodakarensis |
General Information | Comment | Organism |
---|---|---|
evolution | although the eukaryotic counterpart (Trm11) requires another subunit Trm112 for enzymatic activity, the archaeal enzyme does not require a partner subunit. Furthermore, the eukaryotic Trm11-Trm112 complex catalyzes a single methyl transfer reaction and forms only m2G10 in tRNA. The archaeal enzyme is called Trm-G10 or Trm-m22G10 to distinguish it from the eukaryotic enzyme | Thermococcus kodakarensis |
malfunction | the DELTAtrm11 knockout strain grows poorly at 95°C | Thermococcus kodakarensis |
physiological function | tRNA m2G10/m2 2G10 methyltransferase (archaeal Trm11) methylates the 2-amino group in guanosine at position 10 in tRNA and forms N2,N2-dimethylguanosine (m22G10) via intermediate N2-methylguanosine (m2G10). The archaeal Trm11 is required for Thermococcus kodakarensis survival at high temperatures. The m22G10 modification might have effects on stabilization of tRNA and/or correct folding of tRNA at the high temperatures | Thermococcus kodakarensis |