The enzymatic synthesis of N-methylglutamic acid

Shaw, W.V.; Tsai, L.; Stadtman, E.R.; J. Biol. Chem. 241, 935-945 (1966)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

ammonia

competitive with respect to methylamine

Pseudomonas sp.

arsenite

-

Pseudomonas sp.

hydroxylamine

reversible

Pseudomonas sp.

iodoacetate

-

Pseudomonas sp.

N-ethylmaleimide

-

Pseudomonas sp.

p-chloromercuribenzoate

-

Pseudomonas sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

methylamine + L-glutamate

Pseudomonas sp.

involved in one carbon metabolism

NH3 + N-methyl-L-glutamate

-

Pseudomonas sp.

r

methylamine + L-glutamate

Pseudomonas sp. AM1(2006)

involved in one carbon metabolism

NH3 + N-methyl-L-glutamate

-

Pseudomonas sp. AM1(2006)

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

methylamine + L-glutamate

-

485233

Pseudomonas sp.

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp.

r

methylamine + L-glutamate

involved in one carbon metabolism

485233

Pseudomonas sp.

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp.

r

methylamine + L-glutamate

-

485233

Pseudomonas sp. AM1(2006)

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp. AM1(2006)

r

methylamine + L-glutamate

involved in one carbon metabolism

485233

Pseudomonas sp. AM1(2006)

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp. AM1(2006)

r

methylamine + L-glutamate-gamma-methylester

-

485233

Pseudomonas sp.

NH3 + N-methyl-L-glutamate-gamma-methyl ester

-

-

-

r

methylamine + L-glutamate-gamma-methylester

-

485233

Pseudomonas sp. AM1(2006)

NH3 + N-methyl-L-glutamate-gamma-methyl ester

-

-

-

r

methylamine + L-glutamine

-

485233

Pseudomonas sp.

?

-

-

-

r

methylamine + L-glutamine

-

485233

Pseudomonas sp. AM1(2006)

?

-

-

-

r

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

49

-

ammonia

-

Pseudomonas sp.

Inhibitors

Commentary

Organism

Structure

ammonia

competitive with respect to methylamine

Pseudomonas sp.

arsenite

-

Pseudomonas sp.

hydroxylamine

reversible

Pseudomonas sp.

iodoacetate

-

Pseudomonas sp.

N-ethylmaleimide

-

Pseudomonas sp.

p-chloromercuribenzoate

-

Pseudomonas sp.

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

49

-

ammonia

-

Pseudomonas sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

methylamine + L-glutamate

Pseudomonas sp.

involved in one carbon metabolism

NH3 + N-methyl-L-glutamate

-

Pseudomonas sp.

r

methylamine + L-glutamate

Pseudomonas sp. AM1(2006)

involved in one carbon metabolism

NH3 + N-methyl-L-glutamate

-

Pseudomonas sp. AM1(2006)

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

methylamine + L-glutamate

-

485233

Pseudomonas sp.

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp.

r

methylamine + L-glutamate

involved in one carbon metabolism

485233

Pseudomonas sp.

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp.

r

methylamine + L-glutamate

-

485233

Pseudomonas sp. AM1(2006)

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp. AM1(2006)

r

methylamine + L-glutamate

involved in one carbon metabolism

485233

Pseudomonas sp. AM1(2006)

NH3 + N-methyl-L-glutamate

-

485233

Pseudomonas sp. AM1(2006)

r

methylamine + L-glutamate-gamma-methylester

-

485233

Pseudomonas sp.

NH3 + N-methyl-L-glutamate-gamma-methyl ester

-

-

-

r

methylamine + L-glutamate-gamma-methylester

-

485233

Pseudomonas sp. AM1(2006)

NH3 + N-methyl-L-glutamate-gamma-methyl ester

-

-

-

r

methylamine + L-glutamine

-

485233

Pseudomonas sp.

?

-

-

-

r

methylamine + L-glutamine

-

485233

Pseudomonas sp. AM1(2006)

?

-

-

-

r

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

700286

Latypova

Genetics of the glutamate-medi ...

Methyloversatilis universalis, Methyloversatilis universalis FAM5

Mol. Microbiol.

75

426-439

2010

-

-

-

-

-

-

-

-

-

-

-

2

-

5

-

-

1

-

-

-

6

-

2

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

1

-

-

6

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

485237

Bornemann

Flavoenzymes that catalyse rea ...

Pseudomonas sp.

Nat. Prod. Rep.

19

761-772

2002

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

485236

Hersh

N-Methyl-L-glutamate synthase ...

Pseudomonas sp.

Methods Enzymol.

113

36-42

1985

-

-

-

-

-

-

-

-

-

-

2

1

-

1

-

-

1

-

-

-

1

-

12

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

2

1

-

-

-

1

-

-

1

-

12

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

485235

Schuman Jorns

N-Methylglutamate synthetase. ...

Pseudomonas sp., Pseudomonas sp. AM1(2006)

J. Biol. Chem.

250

3620-3628

1975

-

-

-

-

-

-

-

-

-

-

-

2

-

5

-

-

1

-

-

-

3

-

4

-

-

-

-

-

2

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

2

-

-

-

1

-

-

3

-

4

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

485234

Pollock

N-Methylglutamate synthetase. ...

Pseudomonas sp.

J. Biol. Chem.

248

6724-6733

1973

-

-

-

-

-

-

2

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

485233

Shaw

The enzymatic synthesis of N-m ...

Pseudomonas sp., Pseudomonas sp. AM1(2006)

J. Biol. Chem.

241

935-945

1966

-

-

-

-

-

-

6

-

-

-

-

2

-

5

-

-

-

-

-

-

-

-

8

-

-

-

-

-

-

1

1

-

-

1

-

-

-

-

-

-

-

-

-

-

6

1

-

-

-

-

2

-

-

-

-

-

-

-

-

8

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-