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show all sequences of 2.1.1.208

Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes

Mosbacher, T.G.; Bechthold, A.; Schulz, G.E.; J. Mol. Biol. 345, 535-545 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as an N-terminal GST-fusion protein that is subsequently cleaved with thrombin
Streptomyces viridochromogenes
Crystallization (Commentary)
Crystallization
Organism
hanging-drop vapour-diffusion method at 20°C. Crystals obtained belong to space group P4(1)2(1)2 and contained two subunits of AviRb per crystallographic asymmetric unit
Streptomyces viridochromogenes
Engineering
Amino acid exchange
Commentary
Organism
E234A
20% of wild-type activity
Streptomyces viridochromogenes
E234Q
70% of wild-type activity
Streptomyces viridochromogenes
N139A
less than 5% of wild-type activity
Streptomyces viridochromogenes
N262A
less than 5% of wild-type activity
Streptomyces viridochromogenes
N262Q
60% of wild-type activity
Streptomyces viridochromogenes
R145A
less than 5% of wild-type activity
Streptomyces viridochromogenes
T135V
110% of wild-type activity
Streptomyces viridochromogenes
General Stability
General Stability
Organism
AviRb solutions with concentrations above about 0.5 mg/ml are unstable at ionic strengths below 0.3
Streptomyces viridochromogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31602
-
2 * 31602, calculated from sequence
Streptomyces viridochromogenes
60000
-
dynamic light scattering
Streptomyces viridochromogenes
67000
-
gel filtration
Streptomyces viridochromogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
Streptomyces viridochromogenes
AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces viridochromogenes
Q9F5K6
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces viridochromogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
-
709512
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin
709512
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 31602, calculated from sequence
Streptomyces viridochromogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as an N-terminal GST-fusion protein that is subsequently cleaved with thrombin
Streptomyces viridochromogenes
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging-drop vapour-diffusion method at 20°C. Crystals obtained belong to space group P4(1)2(1)2 and contained two subunits of AviRb per crystallographic asymmetric unit
Streptomyces viridochromogenes
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E234A
20% of wild-type activity
Streptomyces viridochromogenes
E234Q
70% of wild-type activity
Streptomyces viridochromogenes
N139A
less than 5% of wild-type activity
Streptomyces viridochromogenes
N262A
less than 5% of wild-type activity
Streptomyces viridochromogenes
N262Q
60% of wild-type activity
Streptomyces viridochromogenes
R145A
less than 5% of wild-type activity
Streptomyces viridochromogenes
T135V
110% of wild-type activity
Streptomyces viridochromogenes
General Stability (protein specific)
General Stability
Organism
AviRb solutions with concentrations above about 0.5 mg/ml are unstable at ionic strengths below 0.3
Streptomyces viridochromogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31602
-
2 * 31602, calculated from sequence
Streptomyces viridochromogenes
60000
-
dynamic light scattering
Streptomyces viridochromogenes
67000
-
gel filtration
Streptomyces viridochromogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
Streptomyces viridochromogenes
AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces viridochromogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
-
709512
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin
709512
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 31602, calculated from sequence
Streptomyces viridochromogenes
Other publictions for EC 2.1.1.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709512
Mosbacher
Structure and function of the ...
Streptomyces viridochromogenes
J. Mol. Biol.
345
535-545
2005
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1
1
7
1
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3
1
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3
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1
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2
1
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1
7
1
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3
1
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1
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2
1
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709992
Treede
The avilamycin resistance dete ...
Streptomyces viridochromogenes
Mol. Microbiol.
49
309-318
2003
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-
-
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-
-
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-
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1
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3
-
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-
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-
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2
-
1
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1
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1
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2
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1
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1
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707163
Weitnauer
An ATP-binding cassette transp ...
Streptomyces viridochromogenes, Streptomyces viridochromogenes Tu57
Antimicrob. Agents Chemother.
45
690-695
2001
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1
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5
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1
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1
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1
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