Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 22000, SDS-PAGE | Pyrococcus abyssi |
22262 | - |
x * 22262, calculated from sequence | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine56 in tRNA | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9UYD1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine56 in tRNA | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA | - |
? | |
S-adenosyl-L-methionine + cytidine56 in tRNA | in vitro, the tRNA ribose 2'-O-methyltransferase aTrm56 specifically catalyses the AdoMet-dependent 2'-O-ribose methylation of cytidine position 56 in tRNA transcripts. In vitro transcribed Escherichia coli tRNALeu2, yeast tRNAPhe, and Pyrococcus abyssi tRNALeu(CAa) tRNAs are methylated with significant initial rates. Mutations of cytosine at position 56 either by uridine or guanine in tRNA abolish methyl incorporation. A tRNA minisubstrate corresponding to the yeast tRNAAsp transcript missing the D stem-loop, is methylated in vitro at a slightly lower efficiency than the wild-type tRNAAsp. A minisubstrate consisting of the T-loop of tRNAAsp closed by a synthetic stem is also tested. This TSL minisubstrate is still methylated in vitro even though the reaction is 4-5fold less efficient than with the wild type substrate. The complete 3D architecture of the tRNA substrate is not required for aTrm56 catalysis. A T stem-loop structure alone is thus a minimal substrate recognized by aTrm56 | Pyrococcus abyssi | S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Pyrococcus abyssi |
? | x * 22262, calculated from sequence | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
aTrm56 | - |
Pyrococcus abyssi |
PAB1040 | - |
Pyrococcus abyssi |
tRNA ribose 2'-O-methyltransferase aTrm56 | - |
Pyrococcus abyssi |