Activating Compound | Comment | Organism | Structure |
---|---|---|---|
queuine | a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium | Dictyostelium discoideum | |
queuine | a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium | Schizosaccharomyces pombe |
Cloned (Comment) | Organism |
---|---|
overexpression of GFP-tagged DnmA in Dictyostelium discoideum | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a DnmA-deletion mutant | Dictyostelium discoideum |
additional information | generation of a Prmt1-deletion mutant | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Dictyostelium discoideum | |
Mg2+ | required | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dictyostelium discoideum | Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs | ? | - |
? | |
additional information | Schizosaccharomyces pombe | Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe | ? | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNA | Dictyostelium discoideum | - |
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | Schizosaccharomyces pombe | - |
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs | Dictyostelium discoideum | ? | - |
? | |
additional information | Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe | Schizosaccharomyces pombe | ? | - |
? | |
additional information | in vitro methylation activity on tRNAAsp and, to a lesser extent, tRNAGlu. No methylation activity with C38-containing tRNAs for histidine, glutamate, and valine | Schizosaccharomyces pombe | ? | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNA | - |
Dictyostelium discoideum | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | - |
Dictyostelium discoideum | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | - |
Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNAGlu | 25% of the activity with tRNAAsp, the activity with tRNAGlu is not affected by the presence of peptone | Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DnmA | - |
Dictyostelium discoideum |
Dnmt2 | - |
Dictyostelium discoideum |
Dnmt2 | - |
Schizosaccharomyces pombe |
PMT1 | - |
Schizosaccharomyces pombe |
pombe methyltransferase 1 | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Dictyostelium discoideum |
22 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Dictyostelium discoideum |
7.5 | - |
assay at | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Dictyostelium discoideum | |
S-adenosyl-L-methionine | - |
Schizosaccharomyces pombe |
Organism | Comment | Expression |
---|---|---|
Schizosaccharomyces pombe | cultivation of Schizosaccharomyces pombe in minimal medium leads to approximately 23% of tRNAAsp methylation | down |
Schizosaccharomyces pombe | methylation is not enhanced by stress conditions such as glucose starvation, heat stress or oxidative stress | additional information |
Schizosaccharomyces pombe | tRNAAsp methylation is induced to 100% when cells are grown in the presence of peptone | up |
General Information | Comment | Organism |
---|---|---|
malfunction | methylation at C11 of tRNASer (GCT) shows an inverse correlation to the presence of Pmt1 and queuine in pmt1-mutant cells in that its level increases from 23 to 37% in the absence of Pmt1 | Schizosaccharomyces pombe |
physiological function | Pmt1 provides in vivo tRNA methylation activity that is strongly controlled by nutritional cues | Schizosaccharomyces pombe |