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Literature summary for 2.1.1.204 extracted from

  • Mueller, M.; Hartmann, M.; Schuster, I.; Bender, S.; Thuering, K.L.; Helm, M.; Katze, J.R.; Nellen, W.; Lyko, F.; Ehrenhofer-Murray, A.E.
    Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine (2015), Nucleic Acids Res., 43, 10952-10962.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
queuine a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium Dictyostelium discoideum
queuine a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of GFP-tagged DnmA in Dictyostelium discoideum Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information generation of a DnmA-deletion mutant Dictyostelium discoideum
additional information generation of a Prmt1-deletion mutant Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Dictyostelium discoideum
Mg2+ required Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Dictyostelium discoideum Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs ?
-
?
additional information Schizosaccharomyces pombe Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe ?
-
?
S-adenosyl-L-methionine + cytosine38 in tRNA Dictyostelium discoideum
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp Schizosaccharomyces pombe
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs Dictyostelium discoideum ?
-
?
additional information Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe Schizosaccharomyces pombe ?
-
?
additional information in vitro methylation activity on tRNAAsp and, to a lesser extent, tRNAGlu. No methylation activity with C38-containing tRNAs for histidine, glutamate, and valine Schizosaccharomyces pombe ?
-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
-
Dictyostelium discoideum S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
Dictyostelium discoideum S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlu 25% of the activity with tRNAAsp, the activity with tRNAGlu is not affected by the presence of peptone Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlu
-
?

Synonyms

Synonyms Comment Organism
DnmA
-
Dictyostelium discoideum
Dnmt2
-
Dictyostelium discoideum
Dnmt2
-
Schizosaccharomyces pombe
PMT1
-
Schizosaccharomyces pombe
pombe methyltransferase 1
-
Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Dictyostelium discoideum
22
-
assay at Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Dictyostelium discoideum
7.5
-
assay at Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Dictyostelium discoideum
S-adenosyl-L-methionine
-
Schizosaccharomyces pombe

Expression

Organism Comment Expression
Schizosaccharomyces pombe cultivation of Schizosaccharomyces pombe in minimal medium leads to approximately 23% of tRNAAsp methylation down
Schizosaccharomyces pombe methylation is not enhanced by stress conditions such as glucose starvation, heat stress or oxidative stress additional information
Schizosaccharomyces pombe tRNAAsp methylation is induced to 100% when cells are grown in the presence of peptone up

General Information

General Information Comment Organism
malfunction methylation at C11 of tRNASer (GCT) shows an inverse correlation to the presence of Pmt1 and queuine in pmt1-mutant cells in that its level increases from 23 to 37% in the absence of Pmt1 Schizosaccharomyces pombe
physiological function Pmt1 provides in vivo tRNA methylation activity that is strongly controlled by nutritional cues Schizosaccharomyces pombe