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Literature summary for 2.1.1.204 extracted from

  • Khoddami, V.; Cairns, B.R.
    Identification of direct targets and modified bases of RNA cytosine methyltransferases (2013), Nat. Biotechnol., 31, 458-464.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.204

Inhibitors

Inhibitors Comment Organism Structure
5-aza-2'-deoxycytidine the catalytic mechanism is disrupted by the suicide inhibitor Homo sapiens
5-azacytidine the catalytic mechanism is disrupted by the suicide inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal ?
-
 ?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
-
 ?
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + cytosine38 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA the enzyme forms a covalent enzyme-substrate intermediate with its target Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal Homo sapiens ?
-
 ?
additional information development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC) bears one DNMT2 target site (C38) Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
-
 ?
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
-
 ?

Synonyms

Synonyms Comment Organism
Dnmt2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the DNMT2 family of cytosine 5-methylation-RNA methyltransferases utilizing only one cysteine in their catalytic pocket Homo sapiens